Abstract
Background: Middle ear effusion is common in brachycephalic dogs with similarities to otitis media with effusion in children. Association with cranial and Eustachian tube morphology and bacterial infection is suspected in both species.
Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.
Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.
Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.
Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.
Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.
Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.
| Original language | English |
|---|---|
| Pages (from-to) | 1454–1463 |
| Number of pages | 9 |
| Journal | Journal of Veterinary Internal Medicine |
| Volume | 34 |
| Early online date | 14 May 2020 |
| DOIs | |
| Publication status | Published - 24 Jul 2020 |
Keywords
- Canine
- Cavalier King Charles spaniel
- Immunohistochemistry
- Middle ear
- Otitis Media With Effusion