Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.

Elspeth Milne; Tim Nuttall; Katia Marioni-Henry; Chiara Piccinelli; Tobias Schwarz; Ali Anvari Azar; Jennifer Harris; Juliet Duncan; Michael Cheeseman

doi:10.1111/jvim.15792

Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.

Elspeth Milne, Tim Nuttall, Katia Marioni-Henry, Chiara Piccinelli, Tobias Schwarz, Ali Anvari Azar, Jennifer Harris, Juliet Duncan, Michael Cheeseman

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Middle ear effusion is common in brachycephalic dogs with similarities to otitis media with effusion in children. Association with cranial and Eustachian tube morphology and bacterial infection is suspected in both species.
Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.
Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.
Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.

Original language	English
Pages (from-to)	1454–1463
Number of pages	9
Journal	Journal of Veterinary Internal Medicine
Volume	34
Early online date	14 May 2020
DOIs	https://doi.org/10.1111/jvim.15792
Publication status	Published - 24 Jul 2020

Keywords

Canine
Cavalier King Charles spaniel
Immunohistochemistry
Middle ear
Otitis Media With Effusion

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10.1111/jvim.15792

Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in anymedium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine
Final published version, 84 KBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Elspeth Milne
- elspeth.milneed.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Veterinary Clinical Pathology
Person: Academic: Research Active

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@article{9be9f1c3bf094e9d9d5916a7c6c1b812,

title = "Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.",

abstract = "Background: Middle ear effusion is common in brachycephalic dogs with similarities to otitis media with effusion in children. Association with cranial and Eustachian tube morphology and bacterial infection is suspected in both species. Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.",

keywords = "Canine, Cavalier King Charles spaniel, Immunohistochemistry, Middle ear, Otitis Media With Effusion",

author = "Elspeth Milne and Tim Nuttall and Katia Marioni-Henry and Chiara Piccinelli and Tobias Schwarz and {Anvari Azar}, Ali and Jennifer Harris and Juliet Duncan and Michael Cheeseman",

year = "2020",

month = jul,

day = "24",

doi = "10.1111/jvim.15792",

language = "English",

volume = "34",

pages = "1454–1463",

journal = "Journal of Veterinary Internal Medicine",

issn = "0891-6640",

publisher = "Wiley-Blackwell",

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Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs. / Milne, Elspeth ; Nuttall, Tim ; Marioni-Henry, Katia; Piccinelli, Chiara; Schwarz, Tobias; Anvari Azar, Ali; Harris, Jennifer; Duncan, Juliet; Cheeseman, Michael.

In: Journal of Veterinary Internal Medicine, Vol. 34, 24.07.2020, p. 1454–1463.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.

AU - Milne, Elspeth

AU - Nuttall, Tim

AU - Marioni-Henry, Katia

AU - Piccinelli, Chiara

AU - Schwarz, Tobias

AU - Anvari Azar, Ali

AU - Harris, Jennifer

AU - Duncan, Juliet

AU - Cheeseman, Michael

PY - 2020/7/24

Y1 - 2020/7/24

N2 - Background: Middle ear effusion is common in brachycephalic dogs with similarities to otitis media with effusion in children. Association with cranial and Eustachian tube morphology and bacterial infection is suspected in both species. Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.

AB - Background: Middle ear effusion is common in brachycephalic dogs with similarities to otitis media with effusion in children. Association with cranial and Eustachian tube morphology and bacterial infection is suspected in both species. Hypothesis/objectives: To determine cytological and bacteriological features of middle ear effusions in dogs, provide information on histological features and further assess the dog as a model of the human disease.Animals: Sixteen live dogs, three post mortem cases of middle ear effusion, and two post mortem controls.Methods: Prospective; clinical investigation using CT, MRI, video-otoscopy, myringotomy; cytological assessment of 30 and bacteriology of 28 effusions; histology and immunohistochemistry (CD3 for T-lymphocytes, Pax5 for B-lymphocytes and MAC387 for macrophages) of 10 middle ear sections.

KW - Canine

KW - Cavalier King Charles spaniel

KW - Immunohistochemistry

KW - Middle ear

KW - Otitis Media With Effusion

U2 - 10.1111/jvim.15792

DO - 10.1111/jvim.15792

M3 - Article

VL - 34

SP - 1454

EP - 1463

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

ER -

Cytological and microbiological characteristics of middle ear effusions in brachycephalic dogs.

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Keywords

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Elspeth Milne

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