Abstract / Description of output
Cytomegalovirus (CMV) infection and reactivation pose a serious threat for patients after haematopoietic stem cell transplantation. We have previously shown that CD8(+) T cells targeting different CMV epitopes correlate with protection at different threshold frequencies in those patients. To investigate if this may relate to a different quality of these cells here we analyse the T-cell receptor diversity of pp50 (245-253)/HLA-A*0101 specific CD8(+) T cells with that of CD8(+) T cells targeting various pp65 peptides. The results from this pilot study show differences in the breadth of the T-cell receptor usage of the different cell populations. We observe for the first time that the T-cell receptor Vβ CDR3 spectratypes used by CMV pp50 (245-253)/HLA-A*0101-specific CD8(+) T cells can reach higher numbers than those used by CD8(+) T cells targeting various pp65 peptides in our patient cohort. This merits further investigation into the effectiveness of the different CMV-specific T cells and their impact on immunosenescence, which is important to eventually define the most useful source of adoptive therapy and monitoring protocols for cytomegalovirus-specific immune responses.
Original language | English |
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Pages (from-to) | 27-39 |
Number of pages | 13 |
Journal | Immunology |
Volume | 135 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2012 |
Keywords / Materials (for Non-textual outputs)
- Viral Proteins
- HLA-A1 Antigen
- Receptors, Antigen, T-Cell, alpha-beta
- DNA-Binding Proteins
- Humans
- Pilot Projects
- Receptor-CD3 Complex, Antigen, T-Cell
- Cytomegalovirus
- Viral Matrix Proteins
- Phosphoproteins
- CD8-Positive T-Lymphocytes
- Adult
- Cohort Studies
- Cytomegalovirus Infections
- Middle Aged
- Peptides
- Female
- Male