Cytoskeletal heart-enriched actin-associated protein (CHAP) is expressed in striated and smooth muscle cells in chick and mouse during embryonic and adult stages

Willemijn van Eldik, Abdelaziz Beqqali, Jantine Monshouwer-Kloots, Christine Mummery, Robert Passier

Research output: Contribution to journalArticlepeer-review

Abstract

We recently identified a new Z-disc protein, CHAP (Cytoskeletal Heart-enriched Actin-associated Protein), which is expressed in striated muscle and plays an important role during embryonic muscle development in mouse and zebrafish. Here, we confirm and further extend these findings by (i) the identification and characterization of the CHAP orthologue in chick and (ii) providing a detailed analysis of CHAP expression in mouse during embryonic and adult stages. Chick CHAP contains a PDZ domain and a nuclear localization signal, resembling the human and mouse CHAPa. CHAP is expressed in the developing heart and somites, as well as muscle precursors of the limb buds in mouse and chick embryos. CHAP expression in heart and skeletal muscle is maintained in adult mice, both in slow and fast muscle fibers. Moreover, besides expression in striated muscle, we demonstrate that CHAP is expressed in smooth muscle cells of aorta, carotid and coronary arteries in adult mice, but not during embryonic development.

Original languageEnglish
Pages (from-to)649-55
Number of pages7
JournalInternational Journal of Developmental Biology
Volume55
Issue number6
DOIs
Publication statusPublished - 2 Sep 2011

Keywords

  • Actin Cytoskeleton
  • Amino Acid Sequence
  • Animals
  • Aorta
  • Avian Proteins
  • Carotid Arteries
  • Cell Differentiation
  • Chick Embryo
  • Chickens
  • Coronary Vessels
  • Cytoskeletal Proteins
  • Embryonic Development
  • Female
  • Heart
  • Limb Buds
  • Mice
  • Microfilament Proteins
  • Molecular Sequence Data
  • Muscle Development
  • Muscle Proteins
  • Muscle, Skeletal
  • Muscle, Smooth
  • Muscle, Striated
  • Myocytes, Cardiac
  • Myocytes, Smooth Muscle
  • Pregnancy
  • Protein Isoforms
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Somites

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