Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

Chiara Martino; Louise Horsfall; Yan Chen; Mayuree Chanasakulniyom; David Paterson; Adrien Brunet; Susan Rosser; Ying-Jin Yuan; Jonathan M. Cooper

doi:10.1002/cbic.201200038

Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

Chiara Martino, Louise Horsfall, Yan Chen, Mayuree Chanasakulniyom, David Paterson, Adrien Brunet, Susan Rosser, Ying-Jin Yuan, Jonathan M. Cooper

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.

Original language	English
Pages (from-to)	792-795
Number of pages	4
Journal	ChemBioChem
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/cbic.201200038
Publication status	Published - 16 Apr 2012

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10.1002/cbic.201200038

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title = "Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models",

abstract = "We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.",

author = "Chiara Martino and Louise Horsfall and Yan Chen and Mayuree Chanasakulniyom and David Paterson and Adrien Brunet and Susan Rosser and Ying-Jin Yuan and Cooper, {Jonathan M.}",

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doi = "10.1002/cbic.201200038",

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Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models. / Martino, Chiara; Horsfall, Louise; Chen, Yan; Chanasakulniyom, Mayuree; Paterson, David; Brunet, Adrien; Rosser, Susan; Yuan, Ying-Jin; Cooper, Jonathan M.

In: ChemBioChem, Vol. 13, No. 6, 16.04.2012, p. 792-795.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

AU - Martino, Chiara

AU - Horsfall, Louise

AU - Chen, Yan

AU - Chanasakulniyom, Mayuree

AU - Paterson, David

AU - Brunet, Adrien

AU - Rosser, Susan

AU - Yuan, Ying-Jin

AU - Cooper, Jonathan M.

PY - 2012/4/16

Y1 - 2012/4/16

N2 - We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.

AB - We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.

U2 - 10.1002/cbic.201200038

DO - 10.1002/cbic.201200038

M3 - Article

VL - 13

SP - 792

EP - 795

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 6

ER -

Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

Abstract

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