Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models

Chiara Martino, Louise Horsfall, Yan Chen, Mayuree Chanasakulniyom, David Paterson, Adrien Brunet, Susan Rosser, Ying-Jin Yuan, Jonathan M. Cooper

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.
Original languageEnglish
Pages (from-to)792-795
Number of pages4
JournalChemBioChem
Volume13
Issue number6
DOIs
Publication statusPublished - 16 Apr 2012

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