Cytotoxic T lymphocyte epitopes identified from a contemporary strain of porcine reproductive and respiratory syndrome virus enhance CD4+CD8+ T, CD8+ T, and γδ T cell responses

Qian M. Cao, Debin Tian, C. Lynn Heffron, Sakthivel Subramaniam, Tanja Opriessnig, Dennis L. Foss, Jay G Calvert, Xiang- Jin Meng

Research output: Contribution to journalArticlepeer-review

Abstract

Immuno-stimulatory class I-restricted cytotoxic T lymphocytes (CTL) epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) are important for vaccine development. In this study we first determined the expression frequency of swine leukocyte antigen (SLA) class I alleles in commercial pigs in the United States. The SLA genotyping result allowed us to predict potential CTL epitopes from a contemporary strain of PRRSV (RFLP 1-7-4) by using bioinformatic tools. The predicted epitopes were then evaluated in an ex vivo stimulation assay with peripheral blood mononuclear cells isolated from pigs experimentally-infected with PRRSV. Using flow-cytometry analysis, we identified a number of immuno-stimulatory CTL epitopes, including two peptides from GP3 and two from Nsp9 that significantly improved both degranulation marker CD107a and IFN-γ production in cytotoxic CD4+CD8+ T cells, CD8+ T cells, and γδ T cells, and two peptides that inhibited IFN-γ production. These CTL epitopes will aid future vaccine development against PRRSV.
Original languageEnglish
JournalVirology
Early online date17 Sep 2019
DOIs
Publication statusE-pub ahead of print - 17 Sep 2019

Keywords

  • Porcine reproductive and respiratory syndrome virus (PRRSV)
  • Cytotoxic T lymphocytes (CTL)
  • Swine leukocyte antigen (SLA) class I
  • Immono-stimulatory CTL epitopes
  • Vaccine

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