Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry

Ruth Andrew; Shazia Khan; Ioannis Stasinopoulos; Colin Church; Natalie Homer; Margaret McLean

doi:10.1016/j.dib.2019.103740

Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry

Ruth Andrew, Shazia Khan, Ioannis Stasinopoulos, Colin Church, Natalie Homer, Margaret McLean

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” [1]. Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.

Original language	English
Journal	Data in brief
Early online date	8 Mar 2019
DOIs	https://doi.org/10.1016/j.dib.2019.103740
Publication status	E-pub ahead of print - 8 Mar 2019

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1-s2.0-S2352340919300897-mainProof, 467 KBLicence: CC BY

Enhanced Risk of Type 2 Diabetes Mellitus following treatment with 5a-Reductase Inhibitors
Andrew, R. & Walker, B.
UK-based charities
1/10/16 → 31/12/17
Project: Research

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title = "Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry",

abstract = "Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” [1]. Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.",

author = "Ruth Andrew and Shazia Khan and Ioannis Stasinopoulos and Colin Church and Natalie Homer and Margaret McLean",

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doi = "10.1016/j.dib.2019.103740",

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journal = "Data in brief",

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T1 - Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry

AU - Andrew, Ruth

AU - Khan, Shazia

AU - Stasinopoulos, Ioannis

AU - Church, Colin

AU - Homer, Natalie

AU - McLean, Margaret

PY - 2019/3/8

Y1 - 2019/3/8

N2 - Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” [1]. Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.

AB - Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” [1]. Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.

U2 - 10.1016/j.dib.2019.103740

DO - 10.1016/j.dib.2019.103740

M3 - Article

SN - 2352-3409

JO - Data in brief

JF - Data in brief

ER -

Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry

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Enhanced Risk of Type 2 Diabetes Mellitus following treatment with 5a-Reductase Inhibitors

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