Data for discriminating dead/live bacteria in homogenous cell suspensions and the effect of insoluble substrates on turbidimetric measurements

Kwabena O. Duedu*, Christopher E. French

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Estimation of bacterial growth by rapid traditional methods such as spectrophometric measurements at 600 nm (OD600) is not applicable for cultures containing insoluble particles in the growth media. Colony counts are the only suitable alternative but these are laborious and not high-throughput. The data presented in this article is related to the research article entitled “Two-colour fluorescence fluorimetric analysis for direct quantification of bacteria and its application in monitoring bacterial growth in cellulose degradation systems” (Duedu and French, 2017) [1]. This data article presents original primary data describing the discrimination of dead/live bacteria in homogenous cell suspensions and how the presence of insoluble substrates affect the turbidity of the suspensions.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalData in brief
Volume12
Early online date8 Apr 2017
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Bacteria growth
  • Fluorimetry
  • Live/dead estimation
  • Propidium iodide
  • SYBR Green I nucleic acid gel stain

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