De novo assembly and annotation of the European abalone Haliotis tuberculata transcriptome

Ewan Harney, Bruno Dubief, Pierre Boudry, Olivier Basuyaux, Markus B Schilhabel, Sylvain Huchette, Christine Paillard, Flavia L D Nunes

Research output: Contribution to journalArticlepeer-review

Abstract

The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5 billion raw paired-end reads, which were assembled into 328,519 contigs. Filtration and clustering produced a transcriptome of 41,099 transcripts, of which 10,626 (25.85%) were annotated with Blast hits, and 7380 of these were annotated with Gene Ontology (GO) terms in Blast2Go. A differential expression analysis comparing all samples from the two life stages identified 5690 and 10,759 transcripts with significantly higher expression in larvae and adult haemolymph respectively. This is the greatest sequencing effort yet in the Haliotis genus, and provides the first high-throughput transcriptomic resource for H. tuberculata.

Original languageEnglish
JournalBMC Genomics
Early online date9 Mar 2016
DOIs
Publication statusE-pub ahead of print - 9 Mar 2016

Keywords

  • RNA-Seq
  • Mollusca
  • Gastropoda
  • Development
  • Vibrio harveyi
  • Climate change

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