Decoding a PNA Encoded Peptide Library by PCR: The Discovery of New Cell Surface Receptor Ligands

Nina Svensen, Juan Jose Diaz-Mochon, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to screen and identify new ligands for cell surface receptors has been a long-standing goal as it might allow targeting of pharmaceutically relevant receptors, such as integrins or G protein coupled receptors. Here, we present a method to amplify hits from a library of PNA-tagged peptides. To this end, human cells, overexpressing either integrins or the CCR6 receptor, were treated with a 10,000 member PNA-encoded peptide library. Extraction of the PNA tags from the surface of the cells was followed by a PNA-tag to DNA translation and amplification enabling decoding of the tags via microarray hybridization. This approach to ligand discovery facilitates screening for differences in surface-receptor ligands and/or receptor expression between different cell types, and opens up a practical approach to PNA-tag amplification.

Original languageEnglish
Pages (from-to)1284-1289
Number of pages6
JournalChemistry and Biology
Volume18
Issue number10
DOIs
Publication statusPublished - 28 Oct 2011

Keywords

  • SMALL-MOLECULE MICROARRAYS
  • COMBINATORIAL CHEMISTRY
  • CHEMICAL LIBRARIES
  • GLIOMA-CELLS
  • NUCLEIC-ACID
  • SELECTION
  • IDENTIFICATION
  • INTEGRIN
  • DELIVERY
  • DESIGN

Fingerprint

Dive into the research topics of 'Decoding a PNA Encoded Peptide Library by PCR: The Discovery of New Cell Surface Receptor Ligands'. Together they form a unique fingerprint.

Cite this