TY - JOUR
T1 - Decorating graphene oxide/nanogold with dextran-based polymer brushes for the construction of ultrasensitive electrochemical enzyme biosensors
AU - Boujakhrout, Abderrahmane
AU - Sanchez, Alfredo
AU - Diez, Paula
AU - Jimenez-Falcao, Sandra
AU - Martinez-Ruiz, Paloma
AU - Pena Alvarez, Miriam
AU - Pingarron, Jose M.
AU - Villalonga, Reynaldo
PY - 2015/3/20
Y1 - 2015/3/20
N2 - A novel strategy was employed to prepare a water-soluble graphene derivative by using dextran-based polymer brushes as solubilizing agents. Graphene oxide was grafted with (3-mercaptopropyl) trimethoxysilane and further decorated with Au nanoparticles. This hybrid nanomaterial was then reduced and anchored with polysaccharide-based polymer brushes by chemisorption of an end-group thiolated dextran derivative on the Au nanoparticles. The resulting hybrid nonmaterial allowed highly stable aqueous dispersions to be obtained, which were used to coat glassy carbon electrodes for the preparation of a model tyrosinase electrochemical biosensor for catechol. The enzyme electrode showed excellent electroanalytical performance with fast response in about 5 s, a linear range of 100 pM–120 nM, a very high sensitivity of 45.9 A M−1 and a very low detection limit of 40 pM for catechol.
AB - A novel strategy was employed to prepare a water-soluble graphene derivative by using dextran-based polymer brushes as solubilizing agents. Graphene oxide was grafted with (3-mercaptopropyl) trimethoxysilane and further decorated with Au nanoparticles. This hybrid nanomaterial was then reduced and anchored with polysaccharide-based polymer brushes by chemisorption of an end-group thiolated dextran derivative on the Au nanoparticles. The resulting hybrid nonmaterial allowed highly stable aqueous dispersions to be obtained, which were used to coat glassy carbon electrodes for the preparation of a model tyrosinase electrochemical biosensor for catechol. The enzyme electrode showed excellent electroanalytical performance with fast response in about 5 s, a linear range of 100 pM–120 nM, a very high sensitivity of 45.9 A M−1 and a very low detection limit of 40 pM for catechol.
U2 - 10.1039/c5tb00451a
DO - 10.1039/c5tb00451a
M3 - Article
JO - Journal of Materials Chemistry B: Materials for biology and medicine
JF - Journal of Materials Chemistry B: Materials for biology and medicine
SN - 2050-750X
ER -