Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Yue Shen, Yun Wang, Tai Chen, Feng Gao, Jianhui Gong, Dariusz Abramczyk, Roy Walker, Hongcui Zhao, Shihong Chen, Wei Luo, Yisha Luo, Carolin A Müller, Adrien Paul-Dubois-Taine, Bonnie Alver, Giovanni Stracquadanio, Leslie A Mitchell, Zhouqing Lou, Yanqun Fan, Baojin Zhou, Bo WenFengji Tan, Yujia Wang, Jin Zi, Zexiong Xie, Bingzhi Li, Kun Yang, Sarah M Richardson, Hui Jiang, Christopher French, Conrad A. Nieduszynski, Romain Koszul, Adele Marston, Yuan Yingjin, Jian Wang, Joel S Bader, Junbiao Dai, Jef D Boeke, Xun Xu, Yizhi Cai, Huanming Yang

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Herein we report the successful design, construction and characterization of a 770 kb synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels, including phenomics, transcriptomics, proteomics, chromosome segregation and replication analysis to provide a thorough and comprehensive analysis of a synthetic chromosome. Our “Trans-Omics” analyses reveal a modest but potentially significant pervasive up-regulation of translational machinery observed in synII is mainly caused by the deletion of 13 tRNAs. By both complementation assays and SCRaMbLE, we targeted and debuged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the HOG response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
Original languageEnglish
Article numbereaaf4791
JournalScience
Volume355
Issue number6329
DOIs
Publication statusPublished - 10 Mar 2017

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