Abstract
Effective network analysis of protein data requires high-quality proteomic datasets. Here, we report a near doubling in coverage of the C. elegans adult proteome, identifying > 11,000 proteins in total with similar to 9,400 proteins reproducibly detected in three biological replicates. Using quantitative mass spectrometry, we identify proteins whose abundances vary with age, revealing a concerted downregulation of proteins involved in specific metabolic pathways and upregulation of cellular stress responses with advancing age. Among these are similar to 30 peroxisomal proteins, including the PRX-5/PEX5 import protein. Functional experiments confirm that protein import into the peroxisome is compromised in vivo in old animals. We also studied the behavior of the set of age-variant proteins in chronologically age-matched, long-lived daf-2 insulin/IGF-1-pathway mutants. Unexpectedly, the levels of many of these age-variant proteins did not scale with extended lifespan. This indicates that, despite their youthful appearance and extended lifespans, not all aspects of aging are reset in these long-lived mutants.
Original language | English |
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Pages (from-to) | 144-159 |
Number of pages | 16 |
Journal | Cell Systems |
Volume | 3 |
Issue number | 2 |
DOIs | |
Publication status | Published - 24 Aug 2016 |
Keywords
- LIFE-SPAN REGULATION
- HEAT-SHOCK FACTOR
- CAENORHABDITIS-ELEGANS
- SACCHAROMYCES-CEREVISIAE
- LONGEVITY
- GENES
- GENETICS
- MASS
- INTERFERENCE
- EXPRESSION