Defining the phenotype of neutrophils following reverse migration in zebrafish

Felix Ellett, Philip M. Elks, Anne L. Robertson, Nikolay V. Ogryzko, Stephen A. Renshaw*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.

Original languageEnglish
Pages (from-to)975-981
Number of pages7
JournalJournal of Leukocyte Biology
Issue number6
Publication statusPublished - 1 Nov 2015


  • Chemotaxis
  • Granulocytes
  • Infection
  • Inflammation
  • Phagocytosis


Dive into the research topics of 'Defining the phenotype of neutrophils following reverse migration in zebrafish'. Together they form a unique fingerprint.

Cite this