Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss

Catriona Paul; Joanne E Povey; Nicola J Lawrence; Jim Selfridge; David W Melton; Philippa T K Saunders

doi:10.1371/journal.pone.0000989

Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss

Catriona Paul, Joanne E Povey, Nicola J Lawrence, Jim Selfridge, David W Melton, Philippa T K Saunders

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Infertility affects approximately 20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating in the male germ line on infertility is poorly understood but may increase miscarriage. Mouse models allow us to investigate how deficiencies in DNA repair/damage response pathways impact on formation and function of male germ cells. We have investigated mice with deletions of ERCC1 (excision repair cross-complementing gene 1), MSH2 (MutS homolog 2, involved in mismatch repair pathway), and p53 (tumour suppressor gene implicated in elimination of germ cells with DNA damage).

Original language	English
Pages (from-to)	e989
Journal	PLoS ONE
Volume	2
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0000989
Publication status	Published - 2007

Keywords / Materials (for Non-textual outputs)

Animals
Chromatin
DNA Damage
DNA Repair
DNA-Binding Proteins
Disease Models, Animal
Endonucleases
Gene Deletion
Germ Cells
Infertility, Male
Male
Mice
MutS Homolog 2 Protein
Spermatocytes
Testis
Tumor Suppressor Protein p53

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10.1371/journal.pone.0000989

Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss.
Copyright: © 2007 Paul et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 3.58 MBLicence: Creative Commons: Attribution (CC-BY)

Cite this

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title = "Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss",

abstract = "Infertility affects approximately 20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating in the male germ line on infertility is poorly understood but may increase miscarriage. Mouse models allow us to investigate how deficiencies in DNA repair/damage response pathways impact on formation and function of male germ cells. We have investigated mice with deletions of ERCC1 (excision repair cross-complementing gene 1), MSH2 (MutS homolog 2, involved in mismatch repair pathway), and p53 (tumour suppressor gene implicated in elimination of germ cells with DNA damage).",

keywords = "Animals, Chromatin, DNA Damage, DNA Repair, DNA-Binding Proteins, Disease Models, Animal, Endonucleases, Gene Deletion, Germ Cells, Infertility, Male, Male, Mice, MutS Homolog 2 Protein, Spermatocytes, Testis, Tumor Suppressor Protein p53",

author = "Catriona Paul and Povey, {Joanne E} and Lawrence, {Nicola J} and Jim Selfridge and Melton, {David W} and Saunders, {Philippa T K}",

year = "2007",

doi = "10.1371/journal.pone.0000989",

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T1 - Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss

AU - Paul, Catriona

AU - Povey, Joanne E

AU - Lawrence, Nicola J

AU - Selfridge, Jim

AU - Melton, David W

AU - Saunders, Philippa T K

PY - 2007

Y1 - 2007

N2 - Infertility affects approximately 20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating in the male germ line on infertility is poorly understood but may increase miscarriage. Mouse models allow us to investigate how deficiencies in DNA repair/damage response pathways impact on formation and function of male germ cells. We have investigated mice with deletions of ERCC1 (excision repair cross-complementing gene 1), MSH2 (MutS homolog 2, involved in mismatch repair pathway), and p53 (tumour suppressor gene implicated in elimination of germ cells with DNA damage).

AB - Infertility affects approximately 20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating in the male germ line on infertility is poorly understood but may increase miscarriage. Mouse models allow us to investigate how deficiencies in DNA repair/damage response pathways impact on formation and function of male germ cells. We have investigated mice with deletions of ERCC1 (excision repair cross-complementing gene 1), MSH2 (MutS homolog 2, involved in mismatch repair pathway), and p53 (tumour suppressor gene implicated in elimination of germ cells with DNA damage).

KW - Animals

KW - Chromatin

KW - DNA Damage

KW - DNA Repair

KW - DNA-Binding Proteins

KW - Disease Models, Animal

KW - Endonucleases

KW - Gene Deletion

KW - Germ Cells

KW - Infertility, Male

KW - Male

KW - Mice

KW - MutS Homolog 2 Protein

KW - Spermatocytes

KW - Testis

KW - Tumor Suppressor Protein p53

U2 - 10.1371/journal.pone.0000989

DO - 10.1371/journal.pone.0000989

M3 - Article

C2 - 17912366

SN - 1932-6203

VL - 2

SP - e989

JO - PLoS ONE

JF - PLoS ONE

IS - 10

ER -

Deletion of genes implicated in protecting the integrity of male germ cells has differential effects on the incidence of DNA breaks and germ cell loss

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Keywords / Materials (for Non-textual outputs)

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