Abstract / Description of output
Since its discovery in the late 90s, Pten has turned out to be one of the most important tumor suppressor genes. Pten loss results in increased activation of PI3K/Akt signaling pathway, which is associated with increased proliferation, survival and neoplastic growth. Here, we have addressed the effects of conditional deletion of Pten in hematopoietic cells by crossing Pten conditional knock-out mice with a knock-in mouse expressing the Cre recombinase in the CD45 locus. CD45 is also known as Leucocyte Common Antigen and it is expressed in virtually all white cells as well as in hematopoietic stem cells. Using a reporter mouse, we demonstrate that CD45-Cre mouse displays recombinase activity in both myeloid and lymphoid cells. However, deletion of Pten in CD45 expressing cells induces development of T-cell acute lymphoblastic leukemia and lymphoma, but not other hematologic malignancies.