TY - JOUR
T1 - Deletion of Pten of CD45-expressing cells leads to development of T-cell lymphoblastic lymphoma but not myeloid malignancies
AU - Medvinsky, Alexander
AU - Hills, David
AU - Mirantes, Cristina
AU - Dosil, Maria Alba
PY - 2016/4/14
Y1 - 2016/4/14
N2 - Since its discovery in the late 90s, Pten has turned out to be one of the most important tumor suppressor genes. Pten loss results in increased activation of PI3K/Akt signaling pathway, which is associated with increased proliferation, survival and neoplastic growth. Here, we have addressed the effects of conditional deletion of Pten in hematopoietic cells by crossing Pten conditional knock-out mice with a knock-in mouse expressing the Cre recombinase in the CD45 locus. CD45 is also known as Leucocyte Common Antigen and it is expressed in virtually all white cells as well as in hematopoietic stem cells. Using a reporter mouse, we demonstrate that CD45-Cre mouse displays recombinase activity in both myeloid and lymphoid cells. However, deletion of Pten in CD45 expressing cells induces development of T-cell acute lymphoblastic leukemia and lymphoma, but not other hematologic malignancies.
AB - Since its discovery in the late 90s, Pten has turned out to be one of the most important tumor suppressor genes. Pten loss results in increased activation of PI3K/Akt signaling pathway, which is associated with increased proliferation, survival and neoplastic growth. Here, we have addressed the effects of conditional deletion of Pten in hematopoietic cells by crossing Pten conditional knock-out mice with a knock-in mouse expressing the Cre recombinase in the CD45 locus. CD45 is also known as Leucocyte Common Antigen and it is expressed in virtually all white cells as well as in hematopoietic stem cells. Using a reporter mouse, we demonstrate that CD45-Cre mouse displays recombinase activity in both myeloid and lymphoid cells. However, deletion of Pten in CD45 expressing cells induces development of T-cell acute lymphoblastic leukemia and lymphoma, but not other hematologic malignancies.
UR - http://www.ncbi.nlm.nih.gov/pubmed/26773036
U2 - 10.1182/blood-2015-09-669036
DO - 10.1182/blood-2015-09-669036
M3 - Article
SN - 0006-4971
VL - 127
SP - 1907
EP - 1911
JO - Blood
JF - Blood
IS - 5
ER -