Demonstration of early functional compromise of bone marrow derived hematopoietic progenitor cells during bovine neonatal pancytopenia through in vitro culture of bone marrow biopsies

Eleanor Laming, Eleonora Melzi, Sandra F E Scholes, Maira Connelly, Charlotte Bell, Keith T Ballingall, Mark P Dagleish, Mara S Rocchi, Kim Willoughby

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine neonatal pancytopenia (BNP) is a syndrome characterised by thrombocytopenia associated with marked bone marrow destruction in calves, widely reported since 2007 in several European countries and since 2011 in New Zealand. The disease is epidemiologically associated with the use of an inactivated bovine virus diarrhoea (BVD) vaccine and is currently considered to be caused by absorption of colostral antibody produced by some vaccinated cows ("BNP dams"). Alloantibodies capable of binding to the leukocyte surface have been detected in BNP dams and antibodies recognising bovine MHC class I and β-2-microglobulin have been detected in vaccinated cattle. In this study, calves were challenged with pooled colostrum collected from BNP dams or from non-BNP dams and their bone marrow hematopoietic progenitor cells (HPC) cultured in vitro from sternal biopsies taken at 24 hours and 6 days post-challenge.
Original languageEnglish
Pages (from-to)599
JournalBMC Research Notes
Volume5
DOIs
Publication statusPublished - 30 Oct 2012

Keywords

  • Animals
  • Animals, Newborn
  • Biological Markers
  • Biopsy
  • Cattle
  • Cell Proliferation
  • Cell Shape
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Colostrum
  • Diarrhea Viruses, Bovine Viral
  • Female
  • Hematopoietic Stem Cells
  • Male
  • Pancytopenia
  • Pilot Projects
  • Pluripotent Stem Cells
  • Pregnancy
  • Syndrome
  • Thrombocytopenia
  • Time Factors
  • Vaccination
  • Vaccines

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