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Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in particular in conjunction with amplification of microbial 16S rRNA genes. Here, the principles of DGGE, based on partial DNA strand separation at a given position in a gradient of chemical denaturant, are described, and an example protocol, optimized for fingerprinting of 200–300 bp fragments of bacterial 16S rRNA genes, is given.
|Title of host publication||DNA Electrophoresis|
|Subtitle of host publication||Methods and Protocols|
|Publication status||Published - 17 Jul 2013|
|Name||Methods in Molecular Biology|
- Denaturing gradient gel electrophoresis
- Single-nucleotide polymorphisms
- 16S rRNA gene analysis
- Microbial communities
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- 2 Finished
1/08/08 → 30/09/09