Denaturing gradient gel electrophoresis

Fiona Strathdee, Andrew Free

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in particular in conjunction with amplification of microbial 16S rRNA genes. Here, the principles of DGGE, based on partial DNA strand separation at a given position in a gradient of chemical denaturant, are described, and an example protocol, optimized for fingerprinting of 200–300 bp fragments of bacterial 16S rRNA genes, is given.
Original languageEnglish
Title of host publicationDNA Electrophoresis
Subtitle of host publicationMethods and Protocols
EditorsSvetlana Makovets
PublisherHumana Press
Pages145-157
Volume1054
ISBN (Electronic)978-1-62703-565-1
ISBN (Print)978-1-62703-564-4
DOIs
Publication statusPublished - 17 Jul 2013

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1054

Keywords

  • Denaturing gradient gel electrophoresis
  • DGGE
  • Single-nucleotide polymorphisms
  • Fingerprinting
  • 16S rRNA gene analysis
  • Microbial communities

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