Projects per year
Abstract / Description of output
In a previous report, keratinocytes were shown to share their gene expression profile with surrounding Langerhans cells (LCs), influencing LC biology. Here, we investigated whether transferred material could substitute gene products in cells subject to Cre/Lox conditional gene deletion. We found that in human Langerin Cre mice, epidermal LCs and CD11b+CD103+ mesenteric DCs overcome gene deletion if the deleted gene was expressed by neighboring cells. The mechanism of material transfer differed from traditional antigen uptake routes, relying on calcium and PI3K, being susceptible to polyguanylic acid inhibition, and remaining unaffected by inflammation. Termed intracellular monitoring, this process was specific to DCs, occurring in all murine DC subsets tested and human monocyte-derived DCs. The transferred material was presented on MHC-I and MHC-II, suggesting a role in regulating immune responses.
Original language | English |
---|---|
Article number | 109119 |
Pages (from-to) | 1-24 |
Number of pages | 24 |
Journal | iScience |
Volume | 27 |
Issue number | 3 |
Early online date | 6 Feb 2024 |
DOIs | |
Publication status | Published - 15 Mar 2024 |
Fingerprint
Dive into the research topics of 'Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Cytosolic Material from Surrounding Cells'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Determining the role of cxcr5-expressing dendritic cells in imune function and tse agent neuroinvasion from the intestine
1/05/09 → 30/09/12
Project: Research