Depth-resolved characterization of diffusion properties within and across minimally-perturbed skin layers

Anthony P. Raphael, Stefano C. Meliga, Xianfeng Chen, Germain J.P. Fernando, Christopher Flaim, Mark A.F. Kendall*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We examine by both experimental and computational means the diffusion of macromolecules through the skin strata (both the epidermis and dermis). Using mouse skin as a test case, we present a novel high-resolution technique to characterize the diffusion properties of heterogeneous biomaterials using 3D imaging of fluorescent probes, precisely-deposited in minimally-perturbed in vivo skin layers. We find the diffusivity of the delivered macromolecules (70 kDa and 2 MDa rhodamine-dextrans) low within the packed cellular arrangement of the epidermis, while gradually increasing (by ~ an order of magnitude) through the dermis - as pores in the fibrillar network enlarge from the papillary to the reticular dermis. Our experimental and computational approaches for investigating the diffusion through skin strata help in the assessment and optimization of controlled delivery of drugs (e.g. vaccines) to specific sites (e.g. antigen presenting cells).

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalJournal of Controlled Release
Volume166
Issue number2
DOIs
Publication statusPublished - 10 Mar 2013

Keywords

  • 3D confocal microscopy
  • Dermis
  • Image processing
  • Macromolecular diffusion
  • Microneedles
  • Rhodamine dextran

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