Derivation of the human embryonic stem cell line RCe009-A (RC-5)

Paul De Sousa, B. Tye, K. Bruce, P. Dand, G. Russell, D.m. Collins, J. Gardner, J.m. Downie, M. Bateman, A. Courtney

Research output: Contribution to journalArticlepeer-review

Abstract

The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
Original languageEnglish
Pages (from-to)418-422
JournalStem Cell Research
Volume16
Issue number2
Early online date16 Feb 2016
DOIs
Publication statusPublished - 1 Mar 2016

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