Derivation of the human embryonic stem cell line RCe009-A (RC-5)

Paul De Sousa; B. Tye; K. Bruce; P. Dand; G. Russell; D.m. Collins; J. Gardner; J.m. Downie; M. Bateman; A. Courtney

doi:10.1016/j.scr.2016.02.030

Derivation of the human embryonic stem cell line RCe009-A (RC-5)

Paul De Sousa, B. Tye, K. Bruce, P. Dand, G. Russell, D.m. Collins, J. Gardner, J.m. Downie, M. Bateman, A. Courtney

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.

Original language	English
Pages (from-to)	418-422
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	16 Feb 2016
DOIs	https://doi.org/10.1016/j.scr.2016.02.030
Publication status	Published - 1 Mar 2016

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10.1016/j.scr.2016.02.030

Derivation of the human embryonic stem cell line RCe009-A (RC-5)
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http://linkinghub.elsevier.com/retrieve/pii/S1873506116000726

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title = "Derivation of the human embryonic stem cell line RCe009-A (RC-5)",

abstract = "The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.",

author = "{De Sousa}, Paul and B. Tye and K. Bruce and P. Dand and G. Russell and D.m. Collins and J. Gardner and J.m. Downie and M. Bateman and A. Courtney",

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doi = "10.1016/j.scr.2016.02.030",

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AU - De Sousa, Paul

AU - Tye, B.

AU - Bruce, K.

AU - Dand, P.

AU - Russell, G.

AU - Collins, D.m.

AU - Gardner, J.

AU - Downie, J.m.

AU - Bateman, M.

AU - Courtney, A.

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AB - The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.

U2 - 10.1016/j.scr.2016.02.030

DO - 10.1016/j.scr.2016.02.030

M3 - Article

SN - 1873-5061

VL - 16

SP - 418

EP - 422

JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Derivation of the human embryonic stem cell line RCe009-A (RC-5)

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