Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics

Andreas Tsiamis, Anthony Buchoux, Stephen Mahon, Anthony J. Walton, Stewart Smith, David J Clarke, Adam A. Stokes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The lab-on-a-chip concept, enabled by microfluidic technology, promises the integration of multiple discrete laboratory techniques into a miniaturised system. Research into microfluidics has generally focused on the development of individual elements of the total system (often with relatively limited functionality), without full consideration for integration into a complete fully optimised and miniaturised system. Typically, the operation of many of the reported lab-on-a-chip devices is dependent on the support of a laboratory framework. In this paper, a demonstrator platform for routine laboratory analysis is designed and built, which fully integrates a number of technologies into a single device with multiple domains such as fluidics, electronics, pneumatics, hydraulics, and photonics. This facilitates the delivery of breakthroughs in research, by incorporating all physical requirements into a single device. To highlight this proposed approach, this demonstrator microsystem acts as a fully integrated biochemical assay reaction system. The resulting design determines enzyme kinetics in an automated process and combines reservoirs, three-dimensional fluidic channels, optical sensing, and electronics in a low-cost, low-power and portable package.
Original languageEnglish
Article number537
Issue number3
Early online date25 Feb 2023
Publication statusE-pub ahead of print - 25 Feb 2023

Keywords / Materials (for Non-textual outputs)

  • fluidics
  • integrated devices
  • integration
  • lab-on-a-chip
  • miniaturised total analysis system
  • optofluidics
  • sensors


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