Design and fabrication of microelectrodes for electrical impedance tomography of cell spheroids

Nadira Jamil*, Stewart Smith, Yunjie Yang, Jiabin Jia, Pierre Bagnaninchi, Eva Gonzalez-Fernandez

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract / Description of output

Three-dimensional (3D) tumour spheroids exhibit great potential as an in vitro for pharmaceutical drug testing. Spheroids represent more physiologically relevant systems compared to 2D cell culture models. In this work, MCF7 spheroids are cultured in Corning® spheroid microplates to investigate cell morphology and proliferation. Platinum microelectrodes designed to enable electrical impedance tomography (EIT) imaging were fabricated on glass substrates. EIT measurements were performed on 250 μm radius circular electrode tips for 0.71 mm MCF7 spheroid at 20 kHz using time difference imaging method. Initial results indicate the capability of EIT to image cell spheroids.

Original languageEnglish
Title of host publicationIECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences
PublisherInstitute of Electrical and Electronics Engineers
Pages426-431
Number of pages6
ISBN (Electronic)9781467377911
DOIs
Publication statusPublished - 6 Feb 2017
Event2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016 - Kuala Lumpur, Malaysia
Duration: 4 Dec 20168 Dec 2016

Publication series

NameIECBES 2016 - IEEE-EMBS Conference on Biomedical Engineering and Sciences

Conference

Conference2016 IEEE-EMBS Conference on Biomedical Engineering and Sciences, IECBES 2016
Country/TerritoryMalaysia
CityKuala Lumpur
Period4/12/168/12/16

Keywords / Materials (for Non-textual outputs)

  • biosensor
  • EIT
  • MCF7
  • microfabrication
  • spheroids culture

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