Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection

J S Aguilar, Peter Ghazal, Edward K Wagner

Research output: Contribution to journalArticlepeer-review

Abstract

The design and construction of a long (75-mer) oligonucleotide-based DNA microarray for herpes simplex virus type 2 transcripts is described. This array is utilized to generate an analysis of HSV-2 transcript abundance as a function of conditions of infection of human cells, and global patterns of HSV-2 transcript abundance are compared with those for HSV-1. General similarities in patterns along with notable differences in specific details are noted. These results reveal a marked conservation in the program of gene activity between phenotypically diverged strains.
Original languageEnglish
Pages (from-to)423-48
Number of pages26
JournalMethods in Molecular Biology
Volume292
Publication statusPublished - 2005

Keywords

  • Chromosome Mapping
  • Herpes Genitalis
  • Herpesvirus 1, Human
  • Herpesvirus 2, Human
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Transcription, Genetic

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