Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Maxime Klausen; Muhammed Ucuncu; Mark Bradley

doi:10.3390/molecules25225239

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Maxime Klausen, Muhammed Ucuncu, Mark Bradley

Research output: Contribution to journal › Review article › peer-review

Abstract

Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2-, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.

Original language	English
Article number	5239
Journal	Molecules (Basel, Switzerland)
Volume	25
Issue number	22
DOIs	https://doi.org/10.3390/molecules25225239
Publication status	Published - 10 Nov 2020

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IRC Next Steps Plus: Photonic Pathogen Theranostics - Point-of-care image guided photonic therapy of bacterial and fungal infection?
Dhaliwal, K. (Principal Investigator), Bradley, M. (Co-investigator), Harrison, E. (Co-investigator), Megia Fernandez, A. (Co-investigator), Mills, B. (Co-investigator), Walsh, T. (Co-investigator) & Williams, G. (Co-investigator)
EPSRC
1/07/19 → 30/06/23
Project: Research
EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M. (Principal Investigator), Bradley, M. (Principal Investigator), Dhaliwal, K. (Co-investigator), Dhaliwal, K. (Co-investigator), Haslett, C. (Co-investigator), Henderson, R. (Co-investigator), Walsh, T. (Co-investigator) & Walsh, T. (Co-investigator)
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research

Cite this

@article{55fb823c84d3420a9c686b6d36677ff7,

title = "Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy",

abstract = "Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2-, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.",

author = "Maxime Klausen and Muhammed Ucuncu and Mark Bradley",

year = "2020",

month = nov,

day = "10",

doi = "10.3390/molecules25225239",

language = "English",

volume = "25",

journal = "Molecules (Basel, Switzerland)",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "22",

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TY - JOUR

T1 - Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

AU - Klausen, Maxime

AU - Ucuncu, Muhammed

AU - Bradley, Mark

PY - 2020/11/10

Y1 - 2020/11/10

N2 - Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2-, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.

AB - Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2-, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.

U2 - 10.3390/molecules25225239

DO - 10.3390/molecules25225239

M3 - Review article

C2 - 33182751

SN - 1420-3049

VL - 25

JO - Molecules (Basel, Switzerland)

JF - Molecules (Basel, Switzerland)

IS - 22

M1 - 5239

ER -

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Abstract

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Projects

IRC Next Steps Plus: Photonic Pathogen Theranostics - Point-of-care image guided photonic therapy of bacterial and fungal infection?

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

Cite this