Designer proteins: applications of genetic code expansion in cell biology

Lloyd Davis; Jason W Chin

doi:10.1038/nrm3286

Designer proteins: applications of genetic code expansion in cell biology

Lloyd Davis, Jason W Chin

Deanery of Biomedical Sciences

Research output: Contribution to journal › Literature review › peer-review

Abstract

Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be site-specifically encoded into proteins in cells and organisms. This is achieved using 'orthogonal' aminoacyl-tRNA synthetase-tRNA pairs that direct amino acid incorporation in response to an amber stop codon (UAG) placed in a gene of interest. Using this approach, it is now possible to study biology in vitro and in vivo with an increased level of molecular precision. This has allowed new biological insights into protein conformational changes, protein interactions, elementary processes in signal transduction and the role of post-translational modifications.

Original language	English
Pages (from-to)	168-82
Number of pages	15
Journal	Nature reviews Molecular cell biology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1038/nrm3286
Publication status	Published - Mar 2012

Keywords

Animals
Genetic Code
Humans
Models, Molecular
Protein Binding
Protein Conformation
Protein Engineering
Protein Interaction Mapping
Protein Processing, Post-Translational
Recombinant Proteins
Signal Transduction

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10.1038/nrm3286

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title = "Designer proteins: applications of genetic code expansion in cell biology",

abstract = "Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be site-specifically encoded into proteins in cells and organisms. This is achieved using 'orthogonal' aminoacyl-tRNA synthetase-tRNA pairs that direct amino acid incorporation in response to an amber stop codon (UAG) placed in a gene of interest. Using this approach, it is now possible to study biology in vitro and in vivo with an increased level of molecular precision. This has allowed new biological insights into protein conformational changes, protein interactions, elementary processes in signal transduction and the role of post-translational modifications.",

keywords = "Animals, Genetic Code, Humans, Models, Molecular, Protein Binding, Protein Conformation, Protein Engineering, Protein Interaction Mapping, Protein Processing, Post-Translational, Recombinant Proteins, Signal Transduction",

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year = "2012",

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doi = "10.1038/nrm3286",

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T2 - applications of genetic code expansion in cell biology

AU - Davis, Lloyd

AU - Chin, Jason W

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N2 - Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be site-specifically encoded into proteins in cells and organisms. This is achieved using 'orthogonal' aminoacyl-tRNA synthetase-tRNA pairs that direct amino acid incorporation in response to an amber stop codon (UAG) placed in a gene of interest. Using this approach, it is now possible to study biology in vitro and in vivo with an increased level of molecular precision. This has allowed new biological insights into protein conformational changes, protein interactions, elementary processes in signal transduction and the role of post-translational modifications.

AB - Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be site-specifically encoded into proteins in cells and organisms. This is achieved using 'orthogonal' aminoacyl-tRNA synthetase-tRNA pairs that direct amino acid incorporation in response to an amber stop codon (UAG) placed in a gene of interest. Using this approach, it is now possible to study biology in vitro and in vivo with an increased level of molecular precision. This has allowed new biological insights into protein conformational changes, protein interactions, elementary processes in signal transduction and the role of post-translational modifications.

KW - Animals

KW - Genetic Code

KW - Humans

KW - Models, Molecular

KW - Protein Binding

KW - Protein Conformation

KW - Protein Engineering

KW - Protein Interaction Mapping

KW - Protein Processing, Post-Translational

KW - Recombinant Proteins

KW - Signal Transduction

U2 - 10.1038/nrm3286

DO - 10.1038/nrm3286

M3 - Literature review

C2 - 22334143

VL - 13

SP - 168

EP - 182

JO - Nature reviews Molecular cell biology

JF - Nature reviews Molecular cell biology

SN - 1471-0072

IS - 3

ER -

Designer proteins: applications of genetic code expansion in cell biology

Abstract

Keywords

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