Desmoglein 3, via an interaction with E-cadherin, is associated with activation of Src

Siu Man Tsang, Li Liu, Muy-Teck Teh, Ann Wheeler, Richard Grose, Ian R Hart, David R Garrod, Farida Fortune, Hong Wan

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Desmoglein 3 (Dsg3), a desmosomal adhesion protein, is expressed in basal and immediate suprabasal layers of skin and across the entire stratified squamous epithelium of oral mucosa. However, increasing evidence suggests that the role of Dsg3 may involve more than just cell-cell adhesion.

METHODOLOGY/PRINCIPAL FINDINGS: To determine possible additional roles of Dsg3 during epithelial cell adhesion we used overexpression of full-length human Dsg3 cDNA, and RNAi-mediated knockdown of this molecule in various epithelial cell types. Overexpression of Dsg3 resulted in a reduced level of E-cadherin but a colocalisation with the E-cadherin-catenin complex of the adherens junctions. Concomitantly these transfected cells exhibited marked migratory capacity and the formation of filopodial protrusions. These latter events are consistent with Src activation and, indeed, Src-specific inhibition reversed these phenotypes. Moreover Dsg3 knockdown, which also reversed the decreased level of E-cadherin, partially blocked Src phosphorylation.

CONCLUSIONS/SIGNIFICANCE: Our data are consistent with the possibility that Dsg3, as an up-stream regulator of Src activity, helps regulate adherens junction formation.

Original languageEnglish
Pages (from-to)e14211
JournalPLoS ONE
Volume5
Issue number12
DOIs
Publication statusPublished - 2010

Keywords

  • Animals
  • COS Cells
  • Cadherins
  • Cell Line, Tumor
  • Cercopithecus aethiops
  • Desmoglein 3
  • Enzyme Activation
  • Epitopes
  • Gene Expression Regulation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Mouth Mucosa
  • Phenotype
  • Protein-Tyrosine Kinases
  • RNA Interference
  • src-Family Kinases

Fingerprint

Dive into the research topics of 'Desmoglein 3, via an interaction with E-cadherin, is associated with activation of Src'. Together they form a unique fingerprint.

Cite this