Detailed tracking of body and leg movements of a freely walking female cricket during phonotaxis

We describe a semi-automated tracking system for insect motion based on commercially available highspeed video cameras and freely available software. We use it to collect detailed three-dimensional kinematic information from female crickets performing free walking phonotaxis towards a calling song stimulus. We mark the insect's joints with small dots of paint and record the movements from underneath with a pair of cameras following the insect as it walks on the transparent floor of an arena. Tracking is done offline, utilizing a kinematic model to constrain the processing. We can obtain the positions and angles of all joints of all legs and six additional body joints, synchronised with stance-swing transitions and the sound pattern, at a 300 Hz frame rate. This data will be used in the further development of models of neural control of phonotaxis. (C) 2011 Elsevier B.V. All rights reserved.