Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA

Priscilla F. Gerber, Qiaoling Gong, Yao Wei Huang, Chong Wang, Derald Holtkamp, Tanja Opriessnig*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. In serum samples from experimentally infected pigs ( n = 35), anti-IgG PEDV antibodies were detected as early as 7 days post-infection. In field serum samples ( n = 239), the diagnostic sensitivity of the S1 ELISA was 100% and the diagnostic specificity was 94%. The S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples ( n = 133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.

Original languageEnglish
Pages (from-to)33-36
JournalVeterinary Journal
Volume202
Issue number1
DOIs
Publication statusPublished - 15 Aug 2014

Keywords

  • Diagnosis
  • ELISA
  • Porcine epidemic diarrhea virus
  • S1 protein
  • Serology

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