Detection of border disease virus in sheep efferent lymphocytes by immunocytochemical and in situ hybridisation techniques

Gary Entrican, A Flack, J. Hopkins, M. Maclean, P F Nettleton

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The prefemoral efferent lymphatics of four sheep persistently infected with a non cytopathic (NCP) isolate of border disease virus (BDV) were cannulated. Recovered lymphocytes were examined for the presence of virus by an immunocytochemical technique employing a pool of monoclonal antibodies which recognise the 120K non-structural polypeptide of NCP BDV. The results revealed that 9.5% of the lymphocytes carried virus antigen. Lymphocytes from two of the sheep were studied by in situ hybridisation using a viral antisense RNA probe complementary to the region of the BDV genome coding for the 120K polypeptide. This showed that 70-80% of the cells were infected, confirming the greater sensitivity of the in situ hybridisation technique.
Original languageEnglish
Pages (from-to)175-80
Number of pages6
JournalArchives of virology. Supplementum
Volume3
DOIs
Publication statusPublished - 1991

Keywords / Materials (for Non-textual outputs)

  • Pestivirus
  • Lymphatic cannulation
  • Monoclonal antibody
  • In Situ Hybridization

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