Abstract / Description of output
The prefemoral efferent lymphatics of four sheep persistently infected with a non cytopathic (NCP) isolate of border disease virus (BDV) were cannulated. Recovered lymphocytes were examined for the presence of virus by an immunocytochemical technique employing a pool of monoclonal antibodies which recognise the 120K non-structural polypeptide of NCP BDV. The results revealed that 9.5% of the lymphocytes carried virus antigen. Lymphocytes from two of the sheep were studied by in situ hybridisation using a viral antisense RNA probe complementary to the region of the BDV genome coding for the 120K polypeptide. This showed that 70-80% of the cells were infected, confirming the greater sensitivity of the in situ hybridisation technique.
Original language | English |
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Pages (from-to) | 175-80 |
Number of pages | 6 |
Journal | Archives of virology. Supplementum |
Volume | 3 |
DOIs | |
Publication status | Published - 1991 |
Keywords / Materials (for Non-textual outputs)
- Pestivirus
- Lymphatic cannulation
- Monoclonal antibody
- In Situ Hybridization