Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques

C Jenkins; M C Pearce; A W Smith; H I Knight; D J Shaw; T Cheasty; G Foster; G J Gunn; G Dougan; H R Smith; G Frankel

doi:10.1046/j.1472-765X.2003.01379.x

Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques

C Jenkins, M C Pearce, A W Smith, H I Knight, D J Shaw, T Cheasty, G Foster, G J Gunn, G Dougan, H R Smith, G Frankel

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes.

Original language	English
Pages (from-to)	207-12
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	37
Issue number	3
DOIs	https://doi.org/10.1046/j.1472-765X.2003.01379.x
Publication status	Published - 2003

Keywords / Materials (for Non-textual outputs)

Agglutination Tests
Animals
Cattle
Cattle Diseases
DNA Probes
Escherichia coli
Escherichia coli Infections
Feces
Female
Immunomagnetic Separation
Polymerase Chain Reaction
Sensitivity and Specificity
Serotyping
Shiga Toxin

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10.1046/j.1472-765X.2003.01379.x

Detection of Escherichia coli serogroups O26, O103, O111 and O145
Copyright 2003 The Society for Applied Microbiology
Final published version, 91.4 KB

http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.2003.01379.x/abstract

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Jenkins, C., Pearce, M. C., Smith, A. W., Knight, H. I., Shaw, D. J., Cheasty, T., Foster, G., Gunn, G. J., Dougan, G., Smith, H. R., & Frankel, G. (2003). Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques. Letters in Applied Microbiology, 37(3), 207-12. https://doi.org/10.1046/j.1472-765X.2003.01379.x

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title = "Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques",

abstract = "The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes.",

keywords = "Agglutination Tests, Animals, Cattle, Cattle Diseases, DNA Probes, Escherichia coli, Escherichia coli Infections, Feces, Female, Immunomagnetic Separation, Polymerase Chain Reaction, Sensitivity and Specificity, Serotyping, Shiga Toxin",

author = "C Jenkins and Pearce, {M C} and Smith, {A W} and Knight, {H I} and Shaw, {D J} and T Cheasty and G Foster and Gunn, {G J} and G Dougan and Smith, {H R} and G Frankel",

year = "2003",

doi = "10.1046/j.1472-765X.2003.01379.x",

language = "English",

volume = "37",

pages = "207--12",

journal = "Letters in Applied Microbiology",

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publisher = "Wiley",

number = "3",

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Jenkins, C, Pearce, MC, Smith, AW, Knight, HI, Shaw, DJ, Cheasty, T, Foster, G, Gunn, GJ, Dougan, G, Smith, HR & Frankel, G 2003, 'Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques', Letters in Applied Microbiology, vol. 37, no. 3, pp. 207-12. https://doi.org/10.1046/j.1472-765X.2003.01379.x

TY - JOUR

T1 - Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques

AU - Jenkins, C

AU - Pearce, M C

AU - Smith, A W

AU - Knight, H I

AU - Shaw, D J

AU - Cheasty, T

AU - Foster, G

AU - Gunn, G J

AU - Dougan, G

AU - Smith, H R

AU - Frankel, G

PY - 2003

Y1 - 2003

N2 - The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes.

AB - The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes.

KW - Agglutination Tests

KW - Animals

KW - Cattle

KW - Cattle Diseases

KW - DNA Probes

KW - Escherichia coli

KW - Escherichia coli Infections

KW - Feces

KW - Female

KW - Immunomagnetic Separation

KW - Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Serotyping

KW - Shiga Toxin

U2 - 10.1046/j.1472-765X.2003.01379.x

DO - 10.1046/j.1472-765X.2003.01379.x

M3 - Article

C2 - 12904221

SN - 0266-8254

VL - 37

SP - 207

EP - 212

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 3

ER -

Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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