Abstract / Description of output
Type I interferons (IFNs) are essential mediators of antiviral responses. These cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies. Despite a fundamental role in health and disease, the direct quantification of type I IFNs has been challenging. Using single-molecule array (Simoa) digital ELISA technology, we recorded attomolar concentrations of IFNα in healthy donors, viral infection, and complex and monogenic interferonopathies. IFNα protein correlated well with functional activity and IFN-stimulated gene expression. High circulating IFNα levels were associated with increased clinical severity in SLE patients, and a study of the cellular source of IFNα protein indicated disease-specific mechanisms. Measurement of IFNα attomolar concentrations by digital ELISA will enhance our understanding of IFN biology and potentially improve the diagnosis and stratification of pathologies associated with IFN dysregulation.
Original language | English |
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Pages (from-to) | 1547-1555 |
Journal | Journal of Experimental Medicine |
Volume | 214 |
Issue number | 5 |
Early online date | 18 Apr 2017 |
DOIs | |
Publication status | Published - 1 May 2017 |
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Yanick Crow
- Deanery of Molecular, Genetic and Population Health Sciences - Chair of Genomic Medicine
- Centre for Genomic and Experimental Medicine
- Edinburgh Neuroscience
Person: Academic: Research Active
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David Hunt
- Deanery of Clinical Sciences - Professor of Neuroinflammatory Medicine
- Centre for Clinical Brain Sciences
- MRC Human Genetics Unit
- Centre for Regenerative Medicine
- Edinburgh Neuroscience
- Euan MacDonald Centre for Motor Neuron Disease Research
- Edinburgh Imaging
- Anne Rowling Regenerative Neurology Clinic
Person: Academic: Research Active