Detection of Mycobacterium leprae DNA in soil: multiple needles in the haystack

Maria Tió-Coma, Thomas Wijnands, Louise Pierneef, Anna Katarina Schilling, Korshed Alam, Johan Chandra Roy, William R Faber, Henk Menke, Toine Pieters, Karen Stevenson, Jan Hendrik Richardus, Annemieke Geluk

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Leprosy is an infectious disease caused by Mycobacterium leprae affecting the skin and nerves. Despite decades of availability of adequate treatment, transmission is unabated and transmission routes are not completely understood. Despite the general assumption that untreated M. leprae infected humans represent the major source of transmission, scarce reports indicate that environmental sources could also play a role as a reservoir. We investigated whether M. leprae DNA is present in soil of regions where leprosy is endemic or areas with possible animal reservoirs (armadillos and red squirrels). Soil samples (n = 73) were collected in Bangladesh, Suriname and the British Isles. Presence of M. leprae DNA was determined by RLEP PCR and genotypes were further identified by Sanger sequencing. M. leprae DNA was identified in 16.0% of soil from houses of leprosy patients (Bangladesh), in 10.7% from armadillos' holes (Suriname) and in 5% from the habitat of lepromatous red squirrels (British Isles). Genotype 1 was found in Bangladesh whilst in Suriname the genotype was 1 or 2. M. leprae DNA can be detected in soil near human and animal sources, suggesting that environmental sources represent (temporary) reservoirs for M. leprae.

Original languageEnglish
Pages (from-to)3165
JournalScientific Reports
Volume9
Issue number1
Early online date28 Feb 2019
DOIs
Publication statusE-pub ahead of print - 28 Feb 2019

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