Detection of variant Creutzfeldt-Jakob disease infectivity in extraneural tissues

M E Bruce, I McConnell, R G Will, J W Ironside

Research output: Contribution to journalArticlepeer-review


Abnormal accumulations of prion protein (PrP) can be detected in the spleen, lymph nodes, and tonsils of patients with variant Creutzfeldt-Jakob disease (vCJD). Therefore, it has been assumed, but not shown, that these tissues harbour infectivity, which in turn presents the potential for iatrogenic spread through surgery. Here, we show and measure levels of infectivity in spleen and tonsil from two patients with vCJD, by bioassay in intracerebrally inoculated RIII mice. Similar bioassays failed to detect infectivity in buffy coat and plasma.

Original languageEnglish
Pages (from-to)208-9
Number of pages2
JournalThe Lancet
Issue number9277
Publication statusPublished - 21 Jul 2001


  • Animals
  • Biological Assay
  • Creutzfeldt-Jakob Syndrome
  • Humans
  • Iatrogenic Disease
  • Mice
  • Palatine Tonsil
  • Prions
  • Spleen


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