Determination of EBV serostatus prior to kidney transplantation: comparison of VIDAS®, LIAISON® and immunofluorescence assays

I Johannessen, M Noel, A Galloway, S Black, M Shearman, C Graham

Research output: Contribution to journalArticlepeer-review

Abstract

Immunosuppression following solid organ transplantation reduces T cell-mediated immune control of Epstein-Barr Virus (EBV), which may then drive development of post-transplant lymphoproliferative disease. Serology plays a key role in determination of risk of outgrowth of such lesions following transplantation. The study compared the VIDAS(®) (bioMérieux) and LIAISON(®) (DiaSorin) enzyme immunoassays (EIAs) and immunofluorescence assays (IFA; MBL-Bion) in the kidney transplantation setting. Sera from 100 live kidney donors [51 males; age range 20-82 years (mean 51.2 years)] and 100 cadaveric kidney recipients [70 males; age range 17-77 years (mean 51.0 years)] were tested. Overall proportional agreement ranged from 96% to 100% for VIDAS(®) and LIAISON(®). Sensitivity ranged from 91% to 100% and 92% to 100% for VIDAS(®)/IFA and LIAISON(®)/IFA, respectively. The VIDAS(®) and LIAISON(®) approaches gave similar results. Such automated random access EIAs are well suited to busy clinical virology laboratories and rapid determination of donor and recipient EBV serostatus prior to transplantation.

Original languageEnglish
Pages (from-to)107-11
Number of pages5
JournalJournal of Virological Methods
Volume203
DOIs
Publication statusPublished - Jul 2014

Keywords

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral
  • Automation, Laboratory
  • Enzyme-Linked Immunosorbent Assay
  • Epstein-Barr Virus Infections
  • Female
  • Fluorescent Antibody Technique
  • Herpesvirus 4, Human
  • Humans
  • Kidney Transplantation
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • Serologic Tests
  • Young Adult

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