Development and characterization of an acellular human pericardial matrix for tissue engineering

Saeed Mirsadraee, Helen E Wilcox, Sotiris A Korossis, John N Kearney, Kevin G Watterson, John Fisher, Eileen Ingham

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

This study aimed to produce an acellular human tissue scaffold with a view to recellularization with autologous cells to produce a tissue-engineered pericardium that can be used as a patch for cardiovascular repair. Human pericardia from cadaveric donors were treated sequentially with hypotonic buffer, SDS in hypotonic buffer, and a nuclease solution. Histological analysis of decellularized matrices showed that the human pericardial tissue retained its histioarchitecture and major structural proteins. There were no whole cells or cell fragments. There were no significant differences in the hydroxyproline (normal and denatured collagen) and glycosaminoglycan content of the tissue before and after decellularization (p > 0.05). There were no significant changes in the ultimate tensile strength after decellularization (p > 0.05). However, there was an increased extensibility when the tissue strips were cut parallel to the visualized collagen bundles (p = 0.005). No indication of contact or extract cytotoxicity was found when using human dermal fibroblasts and A549 cells. In summary, successful decellularization of the human pericardium was achieved producing a biocompatible matrix that retained the major structural components and strength of the native tissue.

Original languageEnglish
Pages (from-to)763-73
Number of pages11
JournalTissue engineering
Issue number4
Publication statusPublished - Apr 2006

Keywords / Materials (for Non-textual outputs)

  • Adenosine Triphosphate
  • Biocompatible Materials
  • Cells, Cultured
  • Collagen
  • Elasticity
  • Epithelial Cells
  • Extracellular Matrix
  • Fibroblasts
  • Glycosaminoglycans
  • Humans
  • Hydroxyproline
  • Pericardium
  • Tensile Strength
  • Tissue Engineering


Dive into the research topics of 'Development and characterization of an acellular human pericardial matrix for tissue engineering'. Together they form a unique fingerprint.

Cite this