Development and optimization of a hybridization technique to type the classical class I and class II B genes of the chicken MHC

Nicola D. Potts, Coraline Bichet, Laurence Merat, Edouard Guitton, Andrew P. Krupa, Terry A. Burke, Lorna J. Kennedy, Gabriele Sorci, Jim Kaufman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The classical class I and class II molecules of the major histocompatibility complex (MHC) play crucial roles in immune responses to infectious pathogens and vaccines as well as being important for autoimmunity, allergy, cancer and reproduction. These classical MHC genes are the most polymorphic known, with roughly 10,000 alleles in humans. In chickens, the MHC (also known as the BF-BL region) determines decisive resistance and susceptibility to infectious pathogens, but relatively few MHC alleles and haplotypes have been described in any detail. We describe a typing protocol for classical chicken class I (BF) and class II B (BLB) genes based on a hybridization method called reference strand-mediated conformational analysis (RSCA). We optimize the various steps, validate the analysis using well-characterized chicken MHC haplotypes, apply the system to type some experimental lines and discover a new chicken class I allele. This work establishes a basis for typing the MHC genes of chickens worldwide and provides an opportunity to correlate with microsatellite and with single nucleotide polymorphism (SNP) typing for approaches involving imputation.

Original languageEnglish
Pages (from-to)647-663
Number of pages17
JournalImmunogenetics
Volume71
Issue number10
DOIs
Publication statusPublished - 25 Nov 2019

Keywords

  • Avian
  • BF1
  • BF2
  • BLB1
  • BLB2
  • Recombination

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