Abstract
A urinary bladder bioreactor was constructed to replicate physiological bladder dynamics. A cyclical low-delivery pressure regulator mimicked filling pressures of the human bladder. Cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds in the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining.
| Original language | English |
|---|---|
| Number of pages | 9 |
| Journal | Methods in Molecular Biology |
| Early online date | 15 Dec 2015 |
| Publication status | E-pub ahead of print - 15 Dec 2015 |
Keywords / Materials (for Non-textual outputs)
- bladder bioreactor
- tissue-engineering
- Regenerative medicine
- urothelial cells