Development of a bladder bioreactor for tissue-engineering in urology

Niall Davis, Anthony Callanan

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A urinary bladder bioreactor was constructed to replicate physiological bladder dynamics. A cyclical low-delivery pressure regulator mimicked filling pressures of the human bladder. Cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds in the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining.
Original languageEnglish
Number of pages9
JournalMethods in Molecular Biology
Early online date15 Dec 2015
Publication statusE-pub ahead of print - 15 Dec 2015

Keywords / Materials (for Non-textual outputs)

  • bladder bioreactor
  • tissue-engineering
  • Regenerative medicine
  • urothelial cells


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