Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

Simon P. Poland, Hanning Mai, Anneliese L. Jarman, Conor Treacy, Ahmet Erdogan, Neil Finlayson, Maddy Parsons, Claire Wells, Robert Henderson, Keisuke Goda (Editor), Kevin K. Tsia (Editor)

Research output: Contribution to conferencePaperpeer-review

Abstract / Description of output

We report the development of a novel confocal line-scanning microscope capable of acquiring video-frame rate TCSPC-based FLIM. The system consists of a one-dimensional laser beam, which is optically conjugated to a 1024×16 single photon avalanche diode(SPAD) based line-imaging CMOS(1), with 23.78 μm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor facilitates the acquisition of up to 16.5 Giga-photon counts/s, enabling operation 66 times faster than our previously reported bespoke high speed FLIM platforms. We will demonstrate its use in live-cell imaging investigating the roles that PAK proteins play in regulation of cytoskeletal dynamics.
Original languageEnglish
Pages40
DOIs
Publication statusPublished - 16 Mar 2023
EventHigh-Speed Biomedical Imaging and Spectroscopy VIII - San Francisco, United States
Duration: 28 Jan 20233 Feb 2023

Conference

ConferenceHigh-Speed Biomedical Imaging and Spectroscopy VIII
Period28/01/233/02/23

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