Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

Simon P. Poland; Hanning Mai; Anneliese L. Jarman; Conor Treacy; Ahmet Erdogan; Neil Finlayson; Maddy Parsons; Claire Wells; Robert Henderson; Keisuke Goda; Kevin K. Tsia

doi:10.1117/12.2650206

Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

Simon P. Poland, Hanning Mai, Anneliese L. Jarman, Conor Treacy, Ahmet Erdogan, Neil Finlayson, Maddy Parsons, Claire Wells, Robert Henderson, Keisuke Goda (Editor), Kevin K. Tsia (Editor)

Research output: Contribution to conference › Paper › peer-review

Abstract / Description of output

We report the development of a novel confocal line-scanning microscope capable of acquiring video-frame rate TCSPC-based FLIM. The system consists of a one-dimensional laser beam, which is optically conjugated to a 1024×16 single photon avalanche diode(SPAD) based line-imaging CMOS(1), with 23.78 μm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor facilitates the acquisition of up to 16.5 Giga-photon counts/s, enabling operation 66 times faster than our previously reported bespoke high speed FLIM platforms. We will demonstrate its use in live-cell imaging investigating the roles that PAK proteins play in regulation of cytoskeletal dynamics.

Original language	English
Pages	40
DOIs	https://doi.org/10.1117/12.2650206
Publication status	Published - 16 Mar 2023
Event	High-Speed Biomedical Imaging and Spectroscopy VIII - San Francisco, United States Duration: 28 Jan 2023 → 3 Feb 2023

Conference

Conference	High-Speed Biomedical Imaging and Spectroscopy VIII
Period	28/01/23 → 3/02/23

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10.1117/12.2650206

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/PC12390/2650206/Development-of-a-high-speed-confocal-line-scanning-FLIM-microscope/10.1117/12.2650206.full

1 Article

Development of a high-speed line-scanning FLIM microscope for biological imaging
Mai, H., Jarman, A., Erdogan, A., Treacy, C., Finlayson, N., Henderson, R. K. & Poland, S., 15 Apr 2023, In: Optics Letters. 48, 8, p. 2042-2045 5 p.
Research output: Contribution to journal › Article › peer-review

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Poland, S. P., Mai, H., Jarman, A. L., Treacy, C., Erdogan, A., Finlayson, N., Parsons, M., Wells, C., Henderson, R., Goda, K. (Ed.), & Tsia, K. K. (Ed.) (2023). Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation). 40. Paper presented at High-Speed Biomedical Imaging and Spectroscopy VIII. https://doi.org/10.1117/12.2650206

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title = "Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)",

abstract = "We report the development of a novel confocal line-scanning microscope capable of acquiring video-frame rate TCSPC-based FLIM. The system consists of a one-dimensional laser beam, which is optically conjugated to a 1024×16 single photon avalanche diode(SPAD) based line-imaging CMOS(1), with 23.78 μm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor facilitates the acquisition of up to 16.5 Giga-photon counts/s, enabling operation 66 times faster than our previously reported bespoke high speed FLIM platforms. We will demonstrate its use in live-cell imaging investigating the roles that PAK proteins play in regulation of cytoskeletal dynamics. ",

author = "Poland, {Simon P.} and Hanning Mai and Jarman, {Anneliese L.} and Conor Treacy and Ahmet Erdogan and Neil Finlayson and Maddy Parsons and Claire Wells and Robert Henderson and Keisuke Goda and Tsia, {Kevin K.}",

year = "2023",

month = mar,

day = "16",

doi = "10.1117/12.2650206",

language = "English",

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Poland, SP, Mai, H, Jarman, AL, Treacy, C , Erdogan, A , Finlayson, N, Parsons, M, Wells, C, Henderson, R, Goda, K (ed.) & Tsia, KK (ed.) 2023, 'Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)', Paper presented at High-Speed Biomedical Imaging and Spectroscopy VIII, 28/01/23 - 3/02/23 pp. 40. https://doi.org/10.1117/12.2650206

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T1 - Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

AU - Poland, Simon P.

AU - Mai, Hanning

AU - Jarman, Anneliese L.

AU - Treacy, Conor

AU - Erdogan, Ahmet

AU - Finlayson, Neil

AU - Parsons, Maddy

AU - Wells, Claire

AU - Henderson, Robert

A2 - Goda, Keisuke

A2 - Tsia, Kevin K.

PY - 2023/3/16

Y1 - 2023/3/16

N2 - We report the development of a novel confocal line-scanning microscope capable of acquiring video-frame rate TCSPC-based FLIM. The system consists of a one-dimensional laser beam, which is optically conjugated to a 1024×16 single photon avalanche diode(SPAD) based line-imaging CMOS(1), with 23.78 μm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor facilitates the acquisition of up to 16.5 Giga-photon counts/s, enabling operation 66 times faster than our previously reported bespoke high speed FLIM platforms. We will demonstrate its use in live-cell imaging investigating the roles that PAK proteins play in regulation of cytoskeletal dynamics.

AB - We report the development of a novel confocal line-scanning microscope capable of acquiring video-frame rate TCSPC-based FLIM. The system consists of a one-dimensional laser beam, which is optically conjugated to a 1024×16 single photon avalanche diode(SPAD) based line-imaging CMOS(1), with 23.78 μm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor facilitates the acquisition of up to 16.5 Giga-photon counts/s, enabling operation 66 times faster than our previously reported bespoke high speed FLIM platforms. We will demonstrate its use in live-cell imaging investigating the roles that PAK proteins play in regulation of cytoskeletal dynamics.

U2 - 10.1117/12.2650206

DO - 10.1117/12.2650206

M3 - Paper

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T2 - High-Speed Biomedical Imaging and Spectroscopy VIII

Y2 - 28 January 2023 through 3 February 2023

ER -

Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

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Research output

Development of a high-speed line-scanning FLIM microscope for biological imaging

Cite this