Development of a high-speed line-scanning FLIM microscope for biological imaging

Hanning Mai, Anneliese Jarman, Ahmet Erdogan, Conor Treacy, Neil Finlayson, Robert K. Henderson, Simon Poland

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.

Original languageEnglish
Pages (from-to)2042-2045
Number of pages5
JournalOptics Letters
Issue number8
Early online date6 Apr 2023
Publication statusPublished - 15 Apr 2023


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