Development of a high-speed line-scanning FLIM microscope for biological imaging

Hanning Mai; Anneliese Jarman; Ahmet Erdogan; Conor Treacy; Neil Finlayson; Robert K. Henderson; Simon Poland

doi:10.1364/OL.482403

Development of a high-speed line-scanning FLIM microscope for biological imaging

Hanning Mai, Anneliese Jarman, Ahmet Erdogan, Conor Treacy, Neil Finlayson, Robert K. Henderson, Simon Poland

Research output: Contribution to journal › Article › peer-review

Abstract

We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.

Original language	English
Pages (from-to)	2042-2045
Number of pages	5
Journal	Optics Letters
Volume	48
Issue number	8
Early online date	6 Apr 2023
DOIs	https://doi.org/10.1364/OL.482403
Publication status	Published - 15 Apr 2023

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10.1364/OL.482403Licence: Creative Commons: Attribution (CC-BY)

482403Accepted author manuscript, 2.59 MB

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EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M., Bradley, M., Dhaliwal, K., Dhaliwal, K., Haslett, C., Henderson, R., Walsh, T. & Walsh, T.
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research

1 Paper

Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)
Poland, S. P., Mai, H., Jarman, A. L., Treacy, C., Erdogan, A., Finlayson, N., Parsons, M., Wells, C., Henderson, R., Goda, K. (ed.) & Tsia, K. K. (ed.), 16 Mar 2023, p. 40.
Research output: Contribution to conference › Paper › peer-review

Cite this

@article{83651e8d172842e088b2e2b4efcc682e,

title = "Development of a high-speed line-scanning FLIM microscope for biological imaging",

abstract = "We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.",

author = "Hanning Mai and Anneliese Jarman and Ahmet Erdogan and Conor Treacy and Neil Finlayson and Henderson, {Robert K.} and Simon Poland",

note = "Funding Information: UK Research and Innovation (MR/T04067X/1); Engineering and Physical Sciences Research Council (EP/K03197X/1); Royal Society (IES\R3\183065). Funding Information: Acknowledgments. This work was supported by a UKRI Future Leaders Fellowship. We would also like to thank ST Microelectronics, Imaging Division, Edinburgh, for their support in manufacturing the CMOS SPAD line array. We acknowledge the help and support of Prof. Simon Ameer-Beg and the Comprehensive Cancer Centre at King{\textquoteright}s College London for providing equipment used in this research. Publisher Copyright: {\textcopyright} 2023 Optica Publishing Group.",

year = "2023",

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doi = "10.1364/OL.482403",

language = "English",

volume = "48",

pages = "2042--2045",

journal = "Optics Letters",

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T1 - Development of a high-speed line-scanning FLIM microscope for biological imaging

AU - Mai, Hanning

AU - Jarman, Anneliese

AU - Erdogan, Ahmet

AU - Treacy, Conor

AU - Finlayson, Neil

AU - Henderson, Robert K.

AU - Poland, Simon

N1 - Funding Information: UK Research and Innovation (MR/T04067X/1); Engineering and Physical Sciences Research Council (EP/K03197X/1); Royal Society (IES\R3\183065). Funding Information: Acknowledgments. This work was supported by a UKRI Future Leaders Fellowship. We would also like to thank ST Microelectronics, Imaging Division, Edinburgh, for their support in manufacturing the CMOS SPAD line array. We acknowledge the help and support of Prof. Simon Ameer-Beg and the Comprehensive Cancer Centre at King’s College London for providing equipment used in this research. Publisher Copyright: © 2023 Optica Publishing Group.

PY - 2023/4/15

Y1 - 2023/4/15

N2 - We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.

AB - We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.

U2 - 10.1364/OL.482403

DO - 10.1364/OL.482403

M3 - Article

SN - 0146-9592

VL - 48

SP - 2042

EP - 2045

JO - Optics Letters

JF - Optics Letters

IS - 8

ER -

Development of a high-speed line-scanning FLIM microscope for biological imaging

Abstract

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Projects

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

Research output

Development of a high-speed confocal line scanning FLIM microscope for live cell imaging (Conference Presentation)

Cite this