Development of a Medium Density Combined-Species SNP Array for Pacific and European Oysters (Crassostrea gigas and Ostrea edulis)

Alejandro P Gutierrez, Frances Turner, Karim Gharbi, Richard Talbot, Natalie R Lowe, Carolina Peñaloza, Mark McCullough, Paulo A Prodöhl, Tim P Bean, Ross D Houston

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Abstract / Description of output

SNP arrays are enabling tools for high-resolution studies of the genetic basis of complex traits in farmed and wild animals. Oysters are of critical importance in many regions from both an ecological and economic perspective, and oyster aquaculture forms a key component of global food security. The aim of our study was to design a combined-species medium density SNP array for Pacific oyster (C. gigas) and European flat oyster (O. edulis), and to test the performance of this array on farmed and wild populations from multiple locations, with a focus on European populations. SNP discovery was carried out by whole genome sequencing of pooled genomic DNA samples from eight C. gigas populations, and RAD Sequencing of 11 geographically diverse O. edulis populations. Nearly 12 million candidate SNPs were discovered and filtered based on several criteria including preference for SNPs segregating in multiple populations and SNPs with monomorphic flanking regions. An Affymetrix Axiom® Custom Array was created and tested on a diverse set of samples (n = 219) showing ~ 27 K high quality SNPs for C. gigas and ~ 11 K high quality SNPs for O. edulis segregating in these populations. A high proportion of SNPs were segregating in each of the populations, and the array was used to detect population structure and levels of linkage disequilibrium. Further testing of the array on three C. gigas nuclear families (n = 165) revealed that the array can be used to clearly distinguish between families both based on identity-by-state clustering parental assignment software. This medium-density, combined-species array will be publicly available through Affymetrix, and will be applied for genome-wide association and evolutionary genetic studies, and for genomic selection in oyster breeding programs.

Original languageEnglish
Pages (from-to)2209-2218
JournalG3: Genes | Genomes | Genetics
Issue number7
Early online date22 May 2017
Publication statusPublished - Jul 2017

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  • Journal Article


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