Development of a quantitative immunoassay for the cystic fibrosis gene

J C Manson, D J Brock

Research output: Contribution to journalArticlepeer-review

Abstract

An antiserum raised in guineapigs showed positive immunoelectrophoretic reaction with sera from 16 of 17 patients with cystic fibrosis, 8 of 9 obligate heterozygotes, and only 1 of 15 normal subjects. The antiserum appeared to be specific to the cystic-fibrosis protein described by Wilson and colleagues. Precipitin peaks were larger with sera from cystic-fibrosis homozygotes than from heterozygotes, suggesting the possibility of a new quantitative biochemical assay for cystic fibrosis.

Original languageEnglish
Pages (from-to)330-1
Number of pages2
JournalThe Lancet
Volume1
Issue number8164
DOIs
Publication statusPublished - 16 Feb 1980

Keywords

  • Animals
  • Antibody Specificity
  • Cystic Fibrosis
  • Genes
  • Guinea Pigs
  • Heterozygote
  • Heterozygote Detection
  • Homozygote
  • Humans
  • Immune Sera
  • Immunoelectrophoresis
  • Immunoglobulin G
  • Isoelectric Focusing
  • Mutation
  • Proteins

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