Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)

Vito Antonio  Mastrochirico Filho; Carolina H. S.  Borges; Milena V.  Freitas; Raquel Belini  Ariede; Fabiana Pilarski; Ricardo Utsunomia; Roberto Carvalheiro; Alejandro Gutierrez; Carolina Penaloza; Jose M.  Yanez; Ross Houston; Diogo Teruo   Hashimoto

doi:10.1186/s12864-020-07090-z

Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)

Vito Antonio Mastrochirico Filho, Carolina H. S. Borges, Milena V. Freitas, Raquel Belini Ariede , Fabiana Pilarski, Ricardo Utsunomia, Roberto Carvalheiro, Alejandro Gutierrez, Carolina Penaloza, Jose M. Yanez, Ross Houston, Diogo Teruo Hashimoto

Research output: Contribution to journal › Article › peer-review

Abstract

Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: binary test survival (TS) and time of death (TD).

Original language	English
Journal	BMC Genomics
Early online date	29 Sept 2020
DOIs	https://doi.org/10.1186/s12864-020-07090-z
Publication status	E-pub ahead of print - 29 Sept 2020

Keywords / Materials (for Non-textual outputs)

Motile Aeromonas septicemia
Disease outbreaks
Resistance QTLs
Fish farming

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10.1186/s12864-020-07090-z

s12864-020-07090-z
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Mastrochirico Filho, V. A., Borges, C. H. S., Freitas, M. V., Ariede , R. B., Pilarski, F., Utsunomia, R., Carvalheiro, R., Gutierrez, A., Penaloza, C., Yanez, J. M., Houston, R., & Hashimoto, D. T. (2020). Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus). BMC Genomics. Advance online publication. https://doi.org/10.1186/s12864-020-07090-z

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title = "Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)",

abstract = "Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: binary test survival (TS) and time of death (TD).",

keywords = "Motile Aeromonas septicemia, Disease outbreaks, Resistance QTLs, Fish farming",

author = "{Mastrochirico Filho}, {Vito Antonio} and Borges, {Carolina H. S.} and Freitas, {Milena V.} and Ariede, {Raquel Belini} and Fabiana Pilarski and Ricardo Utsunomia and Roberto Carvalheiro and Alejandro Gutierrez and Carolina Penaloza and Yanez, {Jose M.} and Ross Houston and Hashimoto, {Diogo Teruo}",

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Mastrochirico Filho, VA, Borges, CHS, Freitas, MV, Ariede , RB, Pilarski, F, Utsunomia, R, Carvalheiro, R, Gutierrez, A, Penaloza, C, Yanez, JM, Houston, R & Hashimoto, DT 2020, 'Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)', BMC Genomics. https://doi.org/10.1186/s12864-020-07090-z

TY - JOUR

T1 - Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)

AU - Mastrochirico Filho, Vito Antonio

AU - Borges, Carolina H. S.

AU - Freitas, Milena V.

AU - Ariede , Raquel Belini

AU - Pilarski, Fabiana

AU - Utsunomia, Ricardo

AU - Carvalheiro, Roberto

AU - Gutierrez, Alejandro

AU - Penaloza, Carolina

AU - Yanez, Jose M.

AU - Houston, Ross

AU - Hashimoto, Diogo Teruo

PY - 2020/9/29

Y1 - 2020/9/29

N2 - Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: binary test survival (TS) and time of death (TD).

AB - Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: binary test survival (TS) and time of death (TD).

KW - Motile Aeromonas septicemia

KW - Disease outbreaks

KW - Resistance QTLs

KW - Fish farming

U2 - 10.1186/s12864-020-07090-z

DO - 10.1186/s12864-020-07090-z

M3 - Article

SN - 1471-2164

JO - BMC Genomics

JF - BMC Genomics

ER -

Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)

Abstract

Keywords / Materials (for Non-textual outputs)

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