Electrowetting on dielectric (EWOD) is a technique for moving small droplets of liquid on a surface. Desorption Electrospray Ionisation (DESI) is capable of ionising protein samples from a surface at ambient temperature and pressure. These two disparate technologies have been combined with high resolution mass spectrometry and bioinformatics to produce a potentially powerful proteomic tool. The power of this technique is in the ability to perform digests and test tube chemistry on a microlitre scale and under computer control. It is expected that both top-down and middle-down proteomic approaches could be performed on samples purified from a complex solution by reversed phase HPLC. Proof of concept has been performed for each step and work is in progress towards system integration.
|Title of host publication||56th ASMS Conference on Mass Spectrometry|
|Publication status||Published - 2008|