Development of Automated Protein Identification by nLC EWOD DESI FT-ICR ECD MS/MS

Adam Stokes, Chris Galloway, Yifan Li, William Parkes, David Dryden, Anthony Walton, Pat Langridge-Smith, Logan Mackay

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electrowetting on dielectric (EWOD) is a technique for moving small droplets of liquid on a surface. Desorption Electrospray Ionisation (DESI) is capable of ionising protein samples from a surface at ambient temperature and pressure. These two disparate technologies have been combined with high resolution mass spectrometry and bioinformatics to produce a potentially powerful proteomic tool. The power of this technique is in the ability to perform digests and test tube chemistry on a microlitre scale and under computer control. It is expected that both top-down and middle-down proteomic approaches could be performed on samples purified from a complex solution by reversed phase HPLC. Proof of concept has been performed for each step and work is in progress towards system integration.
Original languageEnglish
Title of host publication56th ASMS Conference on Mass Spectrometry
Publication statusPublished - 2008

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