TY - JOUR
T1 - Development of biodegradable nanogels for lipase accelerated drug release of 5-aminolevulinic acid
AU - Liu, Xiao
AU - Zhang, Yuan
AU - Zhang, Peng
AU - Ge, Kang
AU - Zhang, Ruzhi
AU - Sun, Yixin
AU - Sheng, Yang
AU - Bradley, Mark
AU - Zhang, Rong
N1 - Funding Information:
We gratefully acknowledge the support from the Jiangsu Innovative & Entrepreneurial Talent group program ( 2017.37 ), ‘ Six talent peaks ’ team project in Jiangsu Province ( SWYY-CXTD-001 ), International Cooperation Project of Changzhou City ( CZ20190019 ) and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/3/17
Y1 - 2023/3/17
N2 - Photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) is an important approach for the treatment of some skin diseases and cancers. A major defect of this approach is that it is difficult for 5-ALA to accumulate around lesions in deeper regions of tissue, resulting in poor conversion to the active fluorophore and photodynamic efficiencies. Because of their targeting and controlled release abilities, nanogel carriers could solve this problem. In this paper, nanogels were prepared by using micro-emulsion polymerization with various biodegradable polyester crosslinkers (L-lactide and ε-caprolactone). The swelling and degradation properties and entrapment efficiency, drug loading and drug release ability of the nanogels were investigated. Nanogels co-cultured with skin cancer cells (A2058) allowed the efficiency of the PDT in vitro to be demonstrated. The results showed that the swelling rate of hydrogels reduced with increasing crosslinker levels, which caused a slow-down in the release of 5-ALA, but lipase accelerated degradation of nanogels increased 5-ALA concentrations in tumor cells and leading to higher PDT efficiency. It was proved by in vivo experiment indicating that the development of skin cancer tissues were efficiently inhibited by the 5-ALA loaded nanogels.
AB - Photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) is an important approach for the treatment of some skin diseases and cancers. A major defect of this approach is that it is difficult for 5-ALA to accumulate around lesions in deeper regions of tissue, resulting in poor conversion to the active fluorophore and photodynamic efficiencies. Because of their targeting and controlled release abilities, nanogel carriers could solve this problem. In this paper, nanogels were prepared by using micro-emulsion polymerization with various biodegradable polyester crosslinkers (L-lactide and ε-caprolactone). The swelling and degradation properties and entrapment efficiency, drug loading and drug release ability of the nanogels were investigated. Nanogels co-cultured with skin cancer cells (A2058) allowed the efficiency of the PDT in vitro to be demonstrated. The results showed that the swelling rate of hydrogels reduced with increasing crosslinker levels, which caused a slow-down in the release of 5-ALA, but lipase accelerated degradation of nanogels increased 5-ALA concentrations in tumor cells and leading to higher PDT efficiency. It was proved by in vivo experiment indicating that the development of skin cancer tissues were efficiently inhibited by the 5-ALA loaded nanogels.
KW - 5-ALA
KW - Biodegradable polyester crosslinker
KW - Controlled drug release
KW - Nanogels
KW - Photodynamic therapy
U2 - 10.1016/j.colsurfb.2023.113268
DO - 10.1016/j.colsurfb.2023.113268
M3 - Article
AN - SCOPUS:85151026828
SN - 0927-7765
VL - 225
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
M1 - 113268
ER -