Development of human chromosome-specific PCR primers for characterization of somatic cell hybrids

C Abbott, S Povey

Research output: Contribution to journalArticlepeer-review

Abstract

The human chromosome complement of somatic cell hybrids must be assessed each time the hybrids are grown in culture. We have developed a panel of human-specific oligonucleotide primers for genes that have been mapped to each of the autosomes and to the X chromosome. These primers enable the human chromosome content of hybrids to be assessed rapidly by PCR. The sequence of the primers is presented together with the appropriate conditions for human DNA-specific amplification for each pair.
Original languageEnglish
Pages (from-to)73-7
Number of pages5
JournalGenomics
Volume9
Issue number1
Publication statusPublished - 1991

Keywords

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • Templates, Genetic

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