Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy

Ilenia Ciani, Holger Schulze, Damion K. Corrigan, Grace Henihan, Gerard Giraud, Jonathan G. Terry, Anthony J. Walton, Ronald Pethig, Peter Ghazal, Jason Crain, Colin J. Campbell, Till T. Bachmann, Andrew R. Mount

Research output: Contribution to journalArticlepeer-review

Abstract

A method for label-free, electrochemical impedance immunosensing for the detection and quantification of three infection biomarkers in both buffer and directly in the defined model matrix of mock wound fluid is demonstrated. Triggering Receptor-1 Expressed on Myeloid cells (TREM-1) and Matrix MetalloPeptidase 9 (MMP-9) are detected via direct assay and N-3-oxo-dodecanoyl-l-HomoSerineLactone (HSL), relevant in bacterial quorum sensing, is detected using a competition assay. Detection is performed with gold screen-printed electrodes modified with a specific thiolated antibody. Detection is achieved in less than 1h straight from mock wound fluid without any extensive sample preparation steps. The limits of detection of 3.3 pM for TREM-1, 1.1 nM for MMP-9 and 1.4 nM for HSL are either near or below the threshold required to indicate infection. A relatively large dynamic range for sensor response is also found, consistent with interaction between neighbouring antibody-antigen complexes in the close-packed surface layer. Together, these three novel electrochemical immunosensors demonstrate viable multi-parameter sensing with the required sensitivity for rapid wound infection detection directly from a clinically relevant specimen.
Original languageEnglish
Pages (from-to)413-418
Number of pages6
JournalBiosensors & Bioelectronics
Volume31
Issue number1
Early online date15 Nov 2011
DOIs
Publication statusPublished - 15 Jan 2012

Keywords

  • Impedimetric biosensor
  • Multiparameter
  • Label free detection
  • Chronic wound infection
  • Immunosensor
  • Mock wound fluid

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