TY - JOUR
T1 - Development of lateral flow assays to detect host proteins in cattle for improved diagnosis of bovine tuberculosis
AU - Khalid, Hamza
AU - Pierneef, Louise
AU - Hooij, Anouk van
AU - Zhou, Zijie
AU - de Jong, Danielle
AU - Fat, Elisa M. Tjon Kon
AU - Connelley, Tim
AU - Hope, Jayne
AU - Corstjens, Paul L. A. M.
AU - Geluk, Annemieke
N1 - Funding Information:
JH and TC were funded by Biotechnology and Biological Sciences Research Council Institute Strategic Programme funding (BB/P013740/1 and BBS/E/D/20002174). AG received funding from the Q.M. Gastmann-Wichers Foundation. HK was funded by a joint Ph.D. studentship from the University of Edinburgh, United Kingdom, and Leiden University Medical Center, The Netherlands.
Publisher Copyright:
Copyright © 2023 Khalid, Pierneef, van Hooij, Zhou, de Jong, Tjon Kon Fat, Connelley, Hope, Corstjens and Geluk.
PY - 2023/8/15
Y1 - 2023/8/15
N2 - Bovine tuberculosis (bTB), caused by
Mycobacterium bovis (
M. bovis) infection in cattle, is an economically devastating chronic disease for livestock worldwide. Efficient disease control measures rely on early and accurate diagnosis using the tuberculin skin test (TST) and interferon-gamma release assays (IGRAs), followed by culling of positive animals. Compromised performance of TST and IGRA, due to BCG vaccination or co-infections with non-tuberculous mycobacteria (NTM), urges improved diagnostics. Lateral flow assays (LFAs) utilizing luminescent upconverting reporter particles (UCP) for quantitative measurement of host biomarkers present an accurate but less equipment- and labor-demanding diagnostic test platform. UCP-LFAs have proven applications for human infectious diseases. Here, we report the development of UCP-LFAs for the detection of six bovine proteins (IFN-γ, IL-2, IL-6, CCL4, CXCL9, and CXCL10), which have been described by ELISA as potential biomarkers to discriminate
M. bovis infected from naïve and BCG-vaccinated cattle. We show that, in line with the ELISA data, the combined PPDb-induced levels of IFN-γ, IL-2, IL-6, CCL4, and CXCL9 determined by UCP-LFAs can discriminate
M. bovis challenged animals from naïve (AUC range: 0.87-1.00) and BCG-vaccinated animals (AUC range: 0.97-1.00) in this cohort. These initial findings can be used to develop a robust and user-friendly multi-biomarker test (MBT) for bTB diagnosis.
AB - Bovine tuberculosis (bTB), caused by
Mycobacterium bovis (
M. bovis) infection in cattle, is an economically devastating chronic disease for livestock worldwide. Efficient disease control measures rely on early and accurate diagnosis using the tuberculin skin test (TST) and interferon-gamma release assays (IGRAs), followed by culling of positive animals. Compromised performance of TST and IGRA, due to BCG vaccination or co-infections with non-tuberculous mycobacteria (NTM), urges improved diagnostics. Lateral flow assays (LFAs) utilizing luminescent upconverting reporter particles (UCP) for quantitative measurement of host biomarkers present an accurate but less equipment- and labor-demanding diagnostic test platform. UCP-LFAs have proven applications for human infectious diseases. Here, we report the development of UCP-LFAs for the detection of six bovine proteins (IFN-γ, IL-2, IL-6, CCL4, CXCL9, and CXCL10), which have been described by ELISA as potential biomarkers to discriminate
M. bovis infected from naïve and BCG-vaccinated cattle. We show that, in line with the ELISA data, the combined PPDb-induced levels of IFN-γ, IL-2, IL-6, CCL4, and CXCL9 determined by UCP-LFAs can discriminate
M. bovis challenged animals from naïve (AUC range: 0.87-1.00) and BCG-vaccinated animals (AUC range: 0.97-1.00) in this cohort. These initial findings can be used to develop a robust and user-friendly multi-biomarker test (MBT) for bTB diagnosis.
KW - biomarkers
KW - bovine tuberculosis
KW - Chemokines
KW - Cytokines
KW - diagnostics
KW - DIVA
KW - upconverting reporter particles
KW - UCP-LFA
U2 - 10.3389/fvets.2023.1193332
DO - 10.3389/fvets.2023.1193332
M3 - Article
C2 - 37655261
SN - 2297-1769
VL - 10
SP - 1
EP - 12
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
ER -