Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus

A Cleland; C Davis; N Adams; C Lycett; L M Jarvis; H Holmes; P Simmonds

doi:10.1046/j.1423-0410.2001.00093.x

Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus

A Cleland, C Davis, N Adams, C Lycett, L M Jarvis, H Holmes, P Simmonds

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In most Western countries, blood donations are routinely screened for hepatitis C virus (HCV) RNA by polymerase chain reaction (PCR) or other nucleic acid tests. We describe the development of a multiplexed assay for human immunodeficiency virus type 1 (HIV-1) and HCV in an internally controlled PCR suitable for large-scale blood donor screening.

Original language	English
Pages (from-to)	93-101
Number of pages	9
Journal	Vox Sanguinis
Volume	81
Issue number	2
DOIs	https://doi.org/10.1046/j.1423-0410.2001.00093.x
Publication status	Published - 2001

Keywords / Materials (for Non-textual outputs)

Conserved Sequence
DNA Primers
Feasibility Studies
HIV Long Terminal Repeat
HIV-1
Hepacivirus
Humans
Polymerase Chain Reaction
RNA, Viral
Sensitivity and Specificity
Sequence Alignment

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10.1046/j.1423-0410.2001.00093.x

Cite this

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title = "Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus",

abstract = "In most Western countries, blood donations are routinely screened for hepatitis C virus (HCV) RNA by polymerase chain reaction (PCR) or other nucleic acid tests. We describe the development of a multiplexed assay for human immunodeficiency virus type 1 (HIV-1) and HCV in an internally controlled PCR suitable for large-scale blood donor screening.",

keywords = "Conserved Sequence, DNA Primers, Feasibility Studies, HIV Long Terminal Repeat, HIV-1, Hepacivirus, Humans, Polymerase Chain Reaction, RNA, Viral, Sensitivity and Specificity, Sequence Alignment",

author = "A Cleland and C Davis and N Adams and C Lycett and Jarvis, {L M} and H Holmes and P Simmonds",

year = "2001",

doi = "10.1046/j.1423-0410.2001.00093.x",

language = "English",

volume = "81",

pages = "93--101",

journal = "Vox Sanguinis",

issn = "0042-9007",

publisher = "Wiley-Blackwell",

number = "2",

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TY - JOUR

T1 - Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus

AU - Cleland, A

AU - Davis, C

AU - Adams, N

AU - Lycett, C

AU - Jarvis, L M

AU - Holmes, H

AU - Simmonds, P

PY - 2001

Y1 - 2001

N2 - In most Western countries, blood donations are routinely screened for hepatitis C virus (HCV) RNA by polymerase chain reaction (PCR) or other nucleic acid tests. We describe the development of a multiplexed assay for human immunodeficiency virus type 1 (HIV-1) and HCV in an internally controlled PCR suitable for large-scale blood donor screening.

AB - In most Western countries, blood donations are routinely screened for hepatitis C virus (HCV) RNA by polymerase chain reaction (PCR) or other nucleic acid tests. We describe the development of a multiplexed assay for human immunodeficiency virus type 1 (HIV-1) and HCV in an internally controlled PCR suitable for large-scale blood donor screening.

KW - Conserved Sequence

KW - DNA Primers

KW - Feasibility Studies

KW - HIV Long Terminal Repeat

KW - HIV-1

KW - Hepacivirus

KW - Humans

KW - Polymerase Chain Reaction

KW - RNA, Viral

KW - Sensitivity and Specificity

KW - Sequence Alignment

U2 - 10.1046/j.1423-0410.2001.00093.x

DO - 10.1046/j.1423-0410.2001.00093.x

M3 - Article

C2 - 11555468

SN - 0042-9007

VL - 81

SP - 93

EP - 101

JO - Vox Sanguinis

JF - Vox Sanguinis

IS - 2

ER -

Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Cite this