Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28

Fiona Powell; Michelle Lawson; Lisa Rothwell; Pete Kaiser

doi:10.1016/j.dci.2008.10.005

Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28

Fiona Powell, Michelle Lawson, Lisa Rothwell, Pete Kaiser

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.

Original language	English
Pages (from-to)	540-546
Number of pages	7
Journal	Developmental and Comparative Immunology
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/j.dci.2008.10.005
Publication status	Published - Apr 2009

Keywords / Materials (for Non-textual outputs)

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antigens, CD28
Antigens, CD4
Antigens, CD8
COS Cells
Cell Line
Cercopithecus aethiops
Chickens
Cloning, Molecular
Cross Reactions
Humans
Mice
Molecular Sequence Data
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Turkeys

Access to Document

10.1016/j.dci.2008.10.005

Development of reagents to study the turkey’s immune response
Copyright 2008 Elsevier Ltd
Final published version, 2.42 MB

http://www.sciencedirect.com/science/article/pii/S0145305X08002309

Ark Genomics
Watson, M., Archibald, A. & Burt, D.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

@article{5bff3bad5ac6410d9af30ee69a106543,

title = "Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28",

abstract = "The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.",

keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD28, Antigens, CD4, Antigens, CD8, COS Cells, Cell Line, Cercopithecus aethiops, Chickens, Cloning, Molecular, Cross Reactions, Humans, Mice, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Turkeys",

author = "Fiona Powell and Michelle Lawson and Lisa Rothwell and Pete Kaiser",

note = "19013189 Powell, Fiona Lawson, Michelle Rothwell, Lisa Kaiser, Pete Biotechnology and Biological Sciences Research Council/United Kingdom Comparative Study Research Support, Non-U.S. Gov't United States Developmental and comparative immunology Dev Comp Immunol. 2009 Apr;33(4):540-6. Epub 2008 Nov 12.",

year = "2009",

month = apr,

doi = "10.1016/j.dci.2008.10.005",

language = "English",

volume = "33",

pages = "540--546",

journal = "Developmental and Comparative Immunology",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Development of reagents to study the turkey's immune response

T2 - Identification and molecular cloning of turkey CD4, CD8alpha and CD28

AU - Powell, Fiona

AU - Lawson, Michelle

AU - Rothwell, Lisa

AU - Kaiser, Pete

N1 - 19013189 Powell, Fiona Lawson, Michelle Rothwell, Lisa Kaiser, Pete Biotechnology and Biological Sciences Research Council/United Kingdom Comparative Study Research Support, Non-U.S. Gov't United States Developmental and comparative immunology Dev Comp Immunol. 2009 Apr;33(4):540-6. Epub 2008 Nov 12.

PY - 2009/4

Y1 - 2009/4

N2 - The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.

AB - The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens, CD28

KW - Antigens, CD4

KW - Antigens, CD8

KW - COS Cells

KW - Cell Line

KW - Cercopithecus aethiops

KW - Chickens

KW - Cloning, Molecular

KW - Cross Reactions

KW - Humans

KW - Mice

KW - Molecular Sequence Data

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Turkeys

U2 - 10.1016/j.dci.2008.10.005

DO - 10.1016/j.dci.2008.10.005

M3 - Article

C2 - 19013189

VL - 33

SP - 540

EP - 546

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 4

ER -

Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

Ark Genomics

Cite this