Development of STR profiling systems for individual identification in wildlife: A case study of the Eurasian badger, Meles meles

Rob Ogden; Nick Dawnay; Ross McEwing

doi:10.1016/j.fsigss.2007.10.224

Development of STR profiling systems for individual identification in wildlife: A case study of the Eurasian badger, Meles meles

Rob Ogden^*, Nick Dawnay, Ross McEwing

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

The application of STR profiling to the individual identification of wildlife is already practised in forensic investigations relating to illegal trade, animal poaching and persecution, however validation of species-specific profiling systems is often limited. Focussing on the Eurasian badger, this study aimed to provide a framework for the application of validation guidelines and match probability statistics to forensic wildlife DNA profiling. Ten STR loci were used to generate 1083 genotypes from 20 UK populations. Markers were individually validated as extensively as possible following SWGDAM guidelines; limitations to this process are discussed. Estimates of population substructure (F_ST = 0.12) and inbreeding (F_IS = 0.11) strongly affected the power of individual discrimination highlighting their importance in wildlife STR profile matching.

Original language	English
Pages (from-to)	612-613
Number of pages	2
Journal	Forensic Science International: Genetics Supplement Series
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigss.2007.10.224
Publication status	Published - 1 Aug 2008

Keywords

Badger
DNA profiling
Individual identification
Validation
Wildlife forensics

Access to Document

10.1016/j.fsigss.2007.10.224

Cite this

@article{0e1c5e8dbc644d16a468fec5e6720ccb,

title = "Development of STR profiling systems for individual identification in wildlife: A case study of the Eurasian badger, Meles meles",

abstract = "The application of STR profiling to the individual identification of wildlife is already practised in forensic investigations relating to illegal trade, animal poaching and persecution, however validation of species-specific profiling systems is often limited. Focussing on the Eurasian badger, this study aimed to provide a framework for the application of validation guidelines and match probability statistics to forensic wildlife DNA profiling. Ten STR loci were used to generate 1083 genotypes from 20 UK populations. Markers were individually validated as extensively as possible following SWGDAM guidelines; limitations to this process are discussed. Estimates of population substructure (FST = 0.12) and inbreeding (FIS = 0.11) strongly affected the power of individual discrimination highlighting their importance in wildlife STR profile matching.",

keywords = "Badger, DNA profiling, Individual identification, Validation, Wildlife forensics",

author = "Rob Ogden and Nick Dawnay and Ross McEwing",

year = "2008",

month = aug,

day = "1",

doi = "10.1016/j.fsigss.2007.10.224",

language = "English",

volume = "1",

pages = "612--613",

journal = "Forensic Science International: Genetics Supplement Series",

issn = "1875-1768",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Development of STR profiling systems for individual identification in wildlife

T2 - A case study of the Eurasian badger, Meles meles

AU - Ogden, Rob

AU - Dawnay, Nick

AU - McEwing, Ross

PY - 2008/8/1

Y1 - 2008/8/1

N2 - The application of STR profiling to the individual identification of wildlife is already practised in forensic investigations relating to illegal trade, animal poaching and persecution, however validation of species-specific profiling systems is often limited. Focussing on the Eurasian badger, this study aimed to provide a framework for the application of validation guidelines and match probability statistics to forensic wildlife DNA profiling. Ten STR loci were used to generate 1083 genotypes from 20 UK populations. Markers were individually validated as extensively as possible following SWGDAM guidelines; limitations to this process are discussed. Estimates of population substructure (FST = 0.12) and inbreeding (FIS = 0.11) strongly affected the power of individual discrimination highlighting their importance in wildlife STR profile matching.

AB - The application of STR profiling to the individual identification of wildlife is already practised in forensic investigations relating to illegal trade, animal poaching and persecution, however validation of species-specific profiling systems is often limited. Focussing on the Eurasian badger, this study aimed to provide a framework for the application of validation guidelines and match probability statistics to forensic wildlife DNA profiling. Ten STR loci were used to generate 1083 genotypes from 20 UK populations. Markers were individually validated as extensively as possible following SWGDAM guidelines; limitations to this process are discussed. Estimates of population substructure (FST = 0.12) and inbreeding (FIS = 0.11) strongly affected the power of individual discrimination highlighting their importance in wildlife STR profile matching.

KW - Badger

KW - DNA profiling

KW - Individual identification

KW - Validation

KW - Wildlife forensics

UR - http://www.scopus.com/inward/record.url?scp=50649103696&partnerID=8YFLogxK

U2 - 10.1016/j.fsigss.2007.10.224

DO - 10.1016/j.fsigss.2007.10.224

M3 - Article

AN - SCOPUS:50649103696

VL - 1

SP - 612

EP - 613

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

SN - 1875-1768

IS - 1

ER -

Development of STR profiling systems for individual identification in wildlife: A case study of the Eurasian badger, Meles meles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this